| 鸭疫里氏杆菌RA JS01菌株的PCR鉴定及生物学特性 |
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| 引用本文： | 张敬峰,李银,刘宇卓,黄欣梅,赵冬敏.鸭疫里氏杆菌RA JS01菌株的PCR鉴定及生物学特性[J].江西农业学报,2011,23(6):111-112,115. |
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| 作者姓名： | 张敬峰 李银 刘宇卓 黄欣梅 赵冬敏 |
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| 作者单位： | 江苏省农业科学院兽医研究所、农业部动物疫病诊断与免疫重点开放实验室、国家兽用生物制品工程技术研究中心,江苏南京,210014 |
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| 基金项目： | 江苏省农业科技自主创新基金“设施养殖场主要疫病防控技术研究与新制品创制” |
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| 摘 要： | 在临床疑似鸭传染性浆膜炎的病鸭组织中分离了1株RA JS01菌株,通过形态观察、生化试验和PCR鉴定,确定该菌株为鸭疫里氏杆菌,结果表明:该菌株对多数抗生素都具有耐药性和较强的致病性;经静脉和足底接种的鸭在接种后24 h时开始死亡,接种96 h时全部死亡;连续传至22代时菌株的生物学特性均未发生变化。
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| 关 键 词： | 鸭疫里氏杆菌 PCR鉴定 生物学特性 |
PCR Identification and Biological Characteristics of Riemerella anatipestifer Strain RA JS01 |
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| ZHANG Jing-feng,LI Yin,LIU Yu-zhuo,HUANG Xin-mei,ZHAO Dong-min.PCR Identification and Biological Characteristics of Riemerella anatipestifer Strain RA JS01[J].Acta Agriculturae Jiangxi,2011,23(6):111-112,115. |
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| Authors: | ZHANG Jing-feng LI Yin LIU Yu-zhuo HUANG Xin-mei ZHAO Dong-min |
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| Institution: | (Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences;Key Laboratory of Animal Diseases Diagnosis and Immunology,Ministry of Agriculture;National Research Center for Veterinary Bio-products Engineering,Nanjing 210014,China) |
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| Abstract: | The bacterium JS01 was isolated from the tissues of clinical sick duck which was suspected to be infected by serositis of duck.This strain was identified to be Riemerella anatipestifer by morphology,biochemical tests and PCR identification.The results showed that the isolated strain was resistant to most of antibiotics,and it had strong pathogenicity.Subculture and preservation of JS01 were studied to provide a basis for vaccine research.Duck began to die 24 h after venous and foot vaccination,and all died 96 h after vaccination.The biological characteristics of the bacterium did not change in the continuous rearing for 22 generations. |
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| Keywords: | Riemerella ranatipestifer PCR identification Biological characteristics |
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