| 利用半定量RT-PCR检测烟碱生物合成的方法 |
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| 引用本文： | 马雷,戚元成,刘卫群.利用半定量RT-PCR检测烟碱生物合成的方法[J].江西农业学报,2009,21(8):12-13. |
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| 作者姓名： | 马雷 戚元成 刘卫群 |
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| 作者单位： | 1. 河南农业大学,烟草学院,河南,郑州,450002
2. 河南农业大学,烟草学院,河南,郑州,450002;河南农业大学,生命科学学院,河南,郑州,450002 |
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| 基金项目： | 国家烟草专卖局重点资助项目 |
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| 摘 要： | 将水培65 d的烟株进行打顶处理,测定打顶和不打顶烟株上部叶的烟碱含量。分别提取根中总RNA,利用腐胺-N-甲基转移酶(PMT)基因特异引物进行actin做内参的半定量RT-PCR,分析打顶前后PMT转录水平。结果表明,上部烟叶烟碱含量和腐胺-N-甲基转移酶基因表达量的变化趋势是一致的。
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| 关 键 词： | 烟碱 腐胺-N-甲基转移酶(PMT) 半定量RT-PCR |
A Method to Determine Nicotine Biosynthesis with Semi-quantitative RT-PCR |
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| MA Lei,QI Yuan-cheng,LIU Wei-qun.A Method to Determine Nicotine Biosynthesis with Semi-quantitative RT-PCR[J].Acta Agriculturae Jiangxi,2009,21(8):12-13. |
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| Authors: | MA Lei QI Yuan-cheng LIU Wei-qun |
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| Institution: | 1.College of Tobacco Science;Henan Agricultural University;Zhengzhou 450002;China;2.College of Life Science;China |
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| Abstract: | The tobacco plants were topped after being water-cultivated for 65 days,and the nicotine content in the top leaf of topped and untopped tobacco plants was examined.Total RNA was extracted respectively from the root of topped and untopped tobacco plants.Expression analysis of putrescine N-methyl transferase(PMT) was carried out by semi-quantitative RT-PCR with the tobacco actin gene as native control.Results showed that the changeable tendency of nicotine content in the top leaf and PMT expression was consis... |
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| Keywords: | Nicotine Putrescine N-methyl transferase Semi-quantitative RT-PCR |
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