| 虎杖的组织培养及高效无性系的建立研究 |
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| 引用本文： | 王宇,方晨,刘洋,徐娜,姜长阳.虎杖的组织培养及高效无性系的建立研究[J].江西农业学报,2009,21(3). |
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| 作者姓名： | 王宇 方晨 刘洋 徐娜 姜长阳 |
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| 作者单位： | 辽宁师范大学,生命科学学院,辽宁,大连,116029 |
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| 摘 要： | 研究了虎杖嫩茎愈伤组织诱导和分化、不定芽的分化和生根、试管苗移栽和扦插及田间栽培所需要的条件,并建立起虎杖嫩茎的高效无性系及快速繁殖技术。结果表明:MS+6-BA0.4 mg/L+NAA1.2 mg/L是诱导形成具有分化能力愈伤组织的理想培养基;MS+AgNO30.8 mg/L+6-BA0.3 mg/L+NAA0.1 mg/L是愈伤组织和不定芽分化的理想培养基;1/3MS+IAA0.4 mg/L+NAA0.1 mg/L是不定芽生根培养和试管苗生根继代培养的理想培养基;炉灰渣是试管苗移栽和扦插的理想基质。移植的试管苗具有长势粗壮旺盛、春天发芽早、秋天枯萎晚、根系发达的性状,其他各种性状保持不变。
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| 关 键 词： | 虎杖 愈伤组织 组织培养 无性系 快速繁殖 |
Tissue Culture and Establishment of High-efficient Clone of Polygonum cuspidatum |
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| WANG Yu,FANG Chen,LIU Yang,XU Na,JIANG Chang-yang.Tissue Culture and Establishment of High-efficient Clone of Polygonum cuspidatum[J].Acta Agriculturae Jiangxi,2009,21(3). |
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| Authors: | WANG Yu FANG Chen LIU Yang XU Na JIANG Chang-yang |
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| Institution: | Biological Scientific College;Liaoning Normal University;Dalian 116029;China |
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| Abstract: | The conditions needed in callus induction and differentiation of tender stem,differentiation and rooting of adventitious bud,transplantation of test-tube seedlings,and field cultivation of Polygonum cuspidatum have been studied,and its regenerative clone and rapid propagation techniques were established.The results showed that the best medium for callus induction was MS+6-BA 0.4 mg/L+NAA 1.2 mg/L.The best medium for differentiation was MS+AgNO3 0.8 mg/L+6-BA 0.3 mg/L+NAA 0.1 mg/L.1/3MS+ IAA 0.4 mg/L+NAA 0.1... |
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| Keywords: | Polygonum cuspidatum Callus Tissue culture Clone Rapid propagation |
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