| 南芥菜花叶病毒分子生物学快速检测方法的研究 |
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| 引用本文： | 吴兴海,邵秀玲,张凤娟,王英超,陈长法,肖西志.南芥菜花叶病毒分子生物学快速检测方法的研究[J].江西农业学报,2007,19(7):34-35. |
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| 作者姓名： | 吴兴海 邵秀玲 张凤娟 王英超 陈长法 肖西志 |
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| 作者单位： | 山东出入境检验检疫局,山东,青岛,266002 |
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| 基金项目： | 国家质检总局植物病原检测专项基金 |
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| 摘 要： | 根据南芥菜花叶病毒(Arabis mosaic Nepovirus)CP基因序列,设计并合成了特异性RT-PCR检测引物,对南芥菜花叶病毒和非南芥菜花叶病毒的感病植物组织进行了PCR扩增反应。结果,南芥菜花叶病毒的感病植物组织的PCR产物均出现364bp的特异性扩增条带,而非南芥菜花叶病毒均未出现扩增条带,证明这对引物具有南芥菜花叶病毒鉴定特异性。将提取的南芥菜花叶病毒总RNA做梯度稀释,测定该检测体系的敏感度,结果表明,此体系最低可检出南芥菜花叶病毒提取的RNA模板浓度为150pg/μL。
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| 关 键 词： | 分子生物学 南芥菜花叶病毒 快速检测 |
| 文章编号： | 1001-8581（2007）07-0034-02 |
| 收稿时间： | 2007-04-06 |
| 修稿时间： | 2007-04-06 |
Study on Rapid Detection of Arabis mosaic Nepovirus by Molecular Biological Method |
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| WU Xing-hai,SHAO Xiu-ling,ZNANG Feng-juan,WANG Ying-chao,CHEN Chang-fa,XIAO Xi-zhi.Study on Rapid Detection of Arabis mosaic Nepovirus by Molecular Biological Method[J].Acta Agriculturae Jiangxi,2007,19(7):34-35. |
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| Authors: | WU Xing-hai SHAO Xiu-ling ZNANG Feng-juan WANG Ying-chao CHEN Chang-fa XIAO Xi-zhi |
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| Institution: | Shandong Exit- Entry Inspection and Quarantine Bureau, Qingdao 266002, China |
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| Abstract: | Specific primers which were designed based on the sequence of CP gene in ArMV by RT-PCR were used for PCR reaction of plant tissue with ArMV and non-ArMV,only the plant tissue with ArMV had the specific amplification of a 364 bp DNA product.The primer was specific to ArMV.The RNA solution of ArMV was diluted by grads to determined sensitivity of the system.The level of detection limit of the primer was 150pg/μL. |
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| Keywords: | PCR Arabis mosaic Nepovirus(ArMV) Rapid detection |
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