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| 番茄叶片再生系统建立的研究 | |
| 引用本文： | 陆云华,张新,马立新.番茄叶片再生系统建立的研究[J].江西农业学报,2005,17(4):40-43. |
| 作者姓名： | 陆云华 张新 马立新 |
| 作者单位： | 1. 湖北大学,生命科学学院分子微生物与基因工程实验室,湖北,武汉,430062;江西省宜春学院生物工程系,江西,宜春,336000 2. 江西省宜春学院生物工程系,江西,宜春,336000 3. 湖北大学,生命科学学院分子微生物与基因工程实验室,湖北,武汉,430062 |
| 基金项目： | 国家“863”项目（2002AA227011）、国家“十五”科技攻关（2001BA70BB）资助项目. |
| 摘 要： | 比较了不同基本培养基、不同激素及其不同浓度组合对4个番茄栽培品种叶片诱导愈伤组织、不定芽分化及其生根的效果.结果表明:MS+6-BA 3.0mg/L+IAA0.2mg/L+蔗糖30 mg/L(pH=5.8)对愈伤组织诱导及诱导愈伤组织分化不定芽的效果最佳;MS+IAA 0.1mg/L+蔗糖20mg/L培养基对生根最好。 |
| 关 键 词： | 番茄 幼叶 组织培养 培养基优化 |
| 文章编号： | 1001-8581（2005）04-0040-04 |
| 收稿时间： | 2005-06-15 |
| 修稿时间： | 2005年6月15日 |
Establishment of Regeneration System of Tomato Leaves |
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| LU Yun -hua,ZHANG Xin,MA Li -xin.Establishment of Regeneration System of Tomato Leaves[J].Acta Agriculturae Jiangxi,2005,17(4):40-43. | |
| Authors: | LU Yun -hua ZHANG Xin MA Li -xin |
| Institution: | 1. Laboratory of Molecular Microbiology and Gene Engineering, College of Life Science, Hubei University , Wuhan 430062 ,China; 2. Biotechnology Department of Yichun University, Yichun 336000 ,China |
| Abstract: | The effects of different media with various hormone concentrations on induction of callus, differentiation of adventitious bud and rooting from 4 different tomato leaves were studied. It showed that callus, shoots and roots could be induced in MS medium. The best medium was MS with 3.0 mg/L 6 - BA plus 0.2 mg/L IAA and 30 mg/L sugar , in which callus grew fast and many embryo calli were produced. The best rooting medium was MS with 0. 1 mg/L IAA and 20 mg/L sugar. |
| Keywords: | Tomato Young leaf Tissue culture Medium optimization |
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