| 鱼类肝细胞分离、原代培养与应用研究综述 |
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| 引用本文： | 贾睿,曹丽萍,丁炜东,杜金梁,徐跑,殷国俊.鱼类肝细胞分离、原代培养与应用研究综述[J].江西农业大学学报,2012(1):147-157. |
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| 作者姓名： | 贾睿 曹丽萍 丁炜东 杜金梁 徐跑 殷国俊 |
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| 作者单位： | 南京农业大学无锡渔业学院;中国水产科学研究院淡水渔业研究中心农业部淡水鱼类遗传育种和养殖生物学重点开放实验室 |
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| 基金项目： | 科技部国际科技合作项目(2009DFA32620);中央级公益性科研院所基本科研业务费专项基金(2011JBFC05);无锡市科技计划(国际科技合作)(CZE00906) |
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| 摘 要： | 鱼类肝细胞分离与原代培养技术已有30多年的历史,目前已有40多种鱼的肝细胞被分离、培养并用于各种研究中。主要讨论鱼类肝细胞的分离与培养方法以及不同条件对细胞生理的影响;概括鱼类肝细胞体外培养在生理学、环境毒理学和药理学等方面的应用研究,旨在为建立更多的鱼类肝细胞原代培养模型并应用于相关的研究领域提供参考依据。
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| 关 键 词： | 鱼类 肝细胞 原代培养 细胞分离 |
Isolation,Primary Culture and Application of Fish Hepatocytes:An overview |
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| JIA Rui,CAO Li-ping,DING Wei-dong,DU Jin-liang,XU Pao,YIN Guo-jun.Isolation,Primary Culture and Application of Fish Hepatocytes:An overview[J].Acta Agriculturae Universitis Jiangxiensis,2012(1):147-157. |
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| Authors: | JIA Rui CAO Li-ping DING Wei-dong DU Jin-liang XU Pao YIN Guo-jun |
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| Institution: | 1,2*(1.Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081,China;2.Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes,Ministry of Agriculture;Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China) |
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| Abstract: | For more than 30 years primary culture technique of fish heaptocytes has been utilized extensively and over 40 fish species studied with respect to the isolation and cultivation of hepatocytes.The review discusses procedures for isolation,primary culture of fish hepatocytes and the influence of different culture conditions on the physiology of the cells;meanwhile the application of ecto-culture of fish heaptocytes culture in physiology of fish,environmental toxicology and pharmacology was sumarized.This paper aims to provide referential information for the establishment of more primary culture models of fish hepatocytes and application in related research. |
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| Keywords: | Fish hepatocytes primary culture cell isolation |
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