| 芦笋花药培养胚状体诱导条件优化的初探 |
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| 引用本文： | 彭新红,周劲松,汤泳萍,尹富强,刘宜柏,陈光宇.芦笋花药培养胚状体诱导条件优化的初探[J].江西农业大学学报,2006,28(1):39-43. |
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| 作者姓名： | 彭新红 周劲松 汤泳萍 尹富强 刘宜柏 陈光宇 |
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| 作者单位： | 1. 江西农业大学,农学院,江西,南昌,330045
2. 江西省农业科学院,生物工程中心,江西,南昌,330200 |
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| 摘 要： | 为建立一种效率较高的芦笋花药培养诱导胚状体的机制,实验以TH IELIM F1为试材,利用正交实验设计L16(45)探讨以MS为基本培养基附加NAA、6-BA、2,4-D、蔗糖、谷氨酰胺等五种因素对芦笋花药培养诱导胚状体的影响。结果表明:NAA是影响芦笋花药培养中诱导胚状体的主要因素,其次是6-BA、蔗糖,而2,4-D、谷氨酰胺的影响较弱。芦笋花药培养诱导胚状体的最优组合是0.01 mg/L NAA、2.0 mg/L 6-BA、1.0 mg/L 2,4-D、50 g/L蔗糖、0 mg/L谷氨酰胺。
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| 关 键 词： | 芦笋 花药培养 胚状体 优化 正交设计 |
| 文章编号： | 1000-2286(2006)01-0039-05 |
| 收稿时间： | 10 17 2005 12:00AM |
| 修稿时间： | 11 16 2005 12:00AM |
Primary Discussion of the Optimization of Culture Conditions for Embryoid Induction of Asparagus(Asparagus officinalis L.)Anther Culture |
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| PENG Xin-hong,ZHOU Jing-song,TANG Yong-ping,YIN Fu-qiang,LIU Yi-bai,CHEN Guang-yu.Primary Discussion of the Optimization of Culture Conditions for Embryoid Induction of Asparagus(Asparagus officinalis L.)Anther Culture[J].Acta Agriculturae Universitis Jiangxiensis,2006,28(1):39-43. |
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| Authors: | PENG Xin-hong ZHOU Jing-song TANG Yong-ping YIN Fu-qiang LIU Yi-bai CHEN Guang-yu |
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| Institution: | 1. College of Agrinomy, Jiangxi Agricuhural University, Nanehang 330045, China; 2. Bioegineering Center, Jiangxi Academy of Agricultural Sciences,Nanchang 330200, China |
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| Abstract: | The aim of this study was to establish an efficient protocol for androgenetic embryoid induction in asparagus anther culture.Cultivar THIELIM F_1 was used in the experiment with MS as the basal medium.Orthogonal arrays of L_(16)(4~5) were adopted to investigate the effect of NAA,6BA,2,4-D,sucrose,glutamine on response of embryoid induction.The results showed that NAA was the most effective element for embryoid induction in asparagus anther culture,followed by 6-BA and sucrose with the least effective for 2,4D and glutamine.The optimized combination was derived with 0.01 mg/LNAA,2.0 mg/L 6-BA,1.0 mg/L 2,4-D,5 g/L sucrose and 0 mg/L glutamine. |
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| Keywords: | asparagus anther culture androgenetic embryoid optimization orthogonal design |
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