| 南昌霉素A产生菌原生质体诱变选育 |
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| 引用本文： | 吴晓玉,涂国全.南昌霉素A产生菌原生质体诱变选育[J].江西农业大学学报,1999(3). |
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| 作者姓名： | 吴晓玉 涂国全 |
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| 作者单位： | 江西农业大学国土资源与环境学院!南昌330045(吴晓玉)，江西农业大学生技中心!南昌330045(涂国全) |
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| 摘 要： | 南昌霉素 Ａ 产生菌———南昌链霉菌（ Ｓｔｒｅｐｔｏｍｙｃｅｓ ｎａｎｃｈａｎｇｅｎｓｉｓ） Ｎ Ａ Ｗ － ３ 菌株经原生质体再生及原生质体紫外线照射处理后，通过摇瓶发酵筛选出高产株 Ｎ Ａ Ｗ－ ３（１） ，其产南昌霉素 Ａ 能力由出发菌株 Ｎ Ａ Ｗ － ３ 的１ ３８３ 单位／ ｍ Ｌ，提高到１ ８９５ 单位／ｍ Ｌ，产素能力提高了３７ ％ 。
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| 关 键 词： | 南昌链霉菌 南昌霉素A 原生质体 诱变 摇瓶发酵 |
A Study on Mutagensis and Selection of Fine Nanchangmycin A Producer by Using Protoplast |
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| Wu Xiaoyu,Tu Guoquan.A Study on Mutagensis and Selection of Fine Nanchangmycin A Producer by Using Protoplast[J].Acta Agriculturae Universitis Jiangxiensis,1999(3). |
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| Authors: | Wu Xiaoyu Tu Guoquan |
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| Institution: | Wu Xiaoyu 1 Tu Guoquan 2 |
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| Abstract: | A more productive strain NAW-3(1), which may yield more Nanchangmycin A, has been selected from Streptomyces Nanchangensis NAW-3 by means of protoplast regeneration and protoplast ultraviolet radiation. The ability of Nanchangmycin A production of the mutant NAW-3(1) in shaking flask fermentation has been raised to 1895 r/mL, 37% higher than that of the strain NAW-3 which yield 1383 r/mL. |
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| Keywords: | Streptomyces nanchangensis Nanchangmycin A protoplast mutate shaking flask fermentation |
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