生殖生长期烟草orf25基因的表达和ATP酶活性分析

为探讨烟草orf25基因与雄性不育性的关系,以处于生殖生长期的烟草雄性不育系MS革新3号及其保持系革新3号为研究材料,运用荧光定量技术测定orf25基因的表达量,同时测定ATP酶活性。结果表明,orf25基因在保持系不同器官中的表达量比不育系的相对较高,而在同一材料中,orf25基因在花器官中的表达量最高,叶片次之,根部表达量相对较低;ATP酶活性也是保持系的比不育系的相对较高,而在同一材料中,花器官中的ATP酶活性也最高,而叶与根之间则无显著差异。相关性分析表明,同一器官中orf25基因的表达量与ATP酶活性之间存在着极显著的正相关。

An Analysis of orf25 Gene and ATPase Activtity of Tobacco during Reproduction Stage

To investigate the relationship between the orf25 gene and male sterility in tobacco,the (CMS) Gexin 3 and Gexin 3,which were in the reproductive growth stage,were used as experimental materials.The re?al?time quantitative PCR(qRT?PCR) was used to analyze the expression patterns of the orf25 gene,and the activity of ATPase was also tested.The results showed that the expression amount of the orf25 gene in Gexin 3 was relatively higher than that in(CMS)Gexin 3,furthermore,the expression of the orf25 gene in flowers was the highest in the same material,that in the leaves took the second place,the expression of orf25 gene in roots was relatively low.The activity of ATPase in the maintainer line was relatively higher than that in its male sterile line,the activity of the ATPase in the flowers was also the highest in the same materials.There was no significant difference of ATPase in leaves and roots.The correlation analysis indicated that the relative expression of orf25 gene had extremely significant positive correlation with the activity of ATPase in the same organ of tobacco.