烟草雄性不育花蕾发育过程中几种物质含量的变化

对烟草雄性不育系和相应的保持系花蕾中游离脯氨酸、可溶性蛋白质和可溶性糖含量进行了分析。结果表明,不育系花蕾的脯氨酸含量在小花蕾阶段与其保持系的相差不大,但从中花蕾开始,保持系花蕾中的脯氨酸含量急剧上升,而不育系花蕾的脯氨酸含量则基本保持稳定,因而不育系花蕾的脯氨酸含量在大花蕾时期比保持系的低得多。不育系花蕾中的可溶性蛋白质含量都比其保持系的低,而且随着花蕾的发育,这种差距还有扩大的趋势。不育系花蕾中可溶性糖含量在小花蕾和中花蕾时期都比其相应的保持系的略高,而在大花蕾阶段则又都比其保持系的略低。所有这些差异与烟草的雄性不育性有一定的关系。

Changes of the Contents of Several Substances During the Development of Male Sterile Flower Buds in Tobacco

The content of free prolin,soluble protein and sugar in flower buds from cytoplasmic male sterile(CMS) lines and their corresponding maintainer lines in tobacco were analyzed.The results showed that the free proline content in the CMS buds were almost equal to that in the maintainer line buds at the small bud stage.However,starting from the mid-size bud stage,the proline content increased rapidly in the maintainer line buds but kept the same in the CMS buds.So the proline content in the CMS buds were much lower than that in the maintainer line buds at the big bud stage.The soluble protein content in the CMS buds was lower than that in the maintainer line buds,and this difference trended to increase along with the bud development.The soluble sugar content in the CMS buds was slightly higher than that in the maintainer line buds at the small and mid-size bud stages.While at the big bud stage,the soluble sugar content in the CMS buds was slightly lower than that in the maintainer line buds.It is suggested that all these differences in substance contents between CMS tobacco and the maintainer line flower buds would be correlated to tobacco male sterility in some extent.