| 土壤宏基因组中硫氧还蛋白还原酶部分序列的克隆 |
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| 引用本文： | 李亚楠,刘晶晶,胡美英,范继巧.土壤宏基因组中硫氧还蛋白还原酶部分序列的克隆[J].江西农业大学学报,2012,34(3):585-589. |
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| 作者姓名： | 李亚楠 刘晶晶 胡美英 范继巧 |
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| 作者单位： | 天然源农药与生物化学教育部重点实验室,华南农业大学昆虫毒理研究室,广东广州510642 |
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| 基金项目： | 国家自然科学基金项目，广东省博士启动基金项目 |
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| 摘 要： | 为探明硫氧还蛋白还原酶(TrxR)在微生物中的保守性,利用宏基因组技术从农药厂废水淤泥中提取环境DNA,并以其为模板直接采用PCR方法进行TrxR基因克隆,最终获得了硫氧还蛋白还原酶基因的部分序列片段。该片段全长476 bp,通过多序列比对及进化树分析,发现其与海杆菌、假单胞菌硫氧还蛋白还原酶基因序列的同源性高达80%以上,氨基酸序列同源性达98%以上,在进化中具有高度的保守性。
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| 关 键 词： | 硫氧还蛋白还原酶 基因克隆 土壤宏基因组 进化树 |
Partial Cloning of Thioredoxin Reductase Gene from Soil Metagenomic DNA |
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| LI Ya-nan
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LIU Jing-jing
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HU Mei-ying
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FAN Ji-qiao.Partial Cloning of Thioredoxin Reductase Gene from Soil Metagenomic DNA[J].Acta Agriculturae Universitis Jiangxiensis,2012,34(3):585-589. |
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| Authors: | LI Ya-nan LIU Jing-jing HU Mei-ying FAN Ji-qiao |
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| Institution: | (Key Laboratory of Natural Pesticide and Chemical Biology,Ministry of Education,Laboratory of Insect Toxicology,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | To prove whether thioredoxin reductase(TrxR) is conservative in the microorganisms, metagenomic technique was used for extraction of the soil metagenomic DNA and cloning of TrxR gene from soil metagenomic DNA by PCR technique in this study.Partial TrxR gene with 476 bp was obteined and analysed by multiple sequence comparison and phylogenetic tree analysis.The results showed that it was more than 80%in homology with TrxR genes from Marine bacterium and Pseudomonas,and more than 98%in amio acid homologous alignments.This shows that TrxR has a high degree of conservativeness in the evolution of microorganisms. |
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| Keywords: | thioredoxin reductase gene cloning soil metagenomic DNA phylogenetic tree |
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