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| 白羽番鸭脂联素基因外显子1与肉质、TC和TG的关联分析 | |
| 引用本文： | 张依裕,徐琪,段修军,张海波,赵文明,孙国波,陈国宏.白羽番鸭脂联素基因外显子1与肉质、TC和TG的关联分析[J].江西农业大学学报,2010,32(1). |
| 作者姓名： | 张依裕 徐琪 段修军 张海波 赵文明 孙国波 陈国宏 |
| 作者单位： | 1. 扬州大学,动物科学与技术学院,江苏,扬州,225009;贵州大学,动物科学学院,贵州,贵阳,550025 2. 扬州大学,动物科学与技术学院,江苏,扬州,225009 3. 江苏省畜牧兽医职业技术学院,江苏,泰州,225300 |
| 基金项目： | 国家科技支撑计划重大专项(2006BDA01A09);;国家科技支撑计划重点项目(2006BDA14B06);;现代农业产业技术体系建设专项资金(nycytx-45-04)联合资助 |
| 摘 要： | 采用PCR-SSCP法对白羽番鸭脂联素基因外显子1进行SNP检测,并分析SNP对肉质、血清总胆固醇(TC)和甘油三酯(TG)的遗传效应。结果表明,检测到3种基因型(AA、AB和BB)和2个等位基因(A和B),AA和A分别为优势基因型和优势等位基因,序列比对发现在外显子1存在1个SNP位点:A167G,为沉默突变,该位点在白羽番鸭群体中表现为中度多态,处于Hardy-Weinberg平衡状态(P>0.05);最小二乘法分析表明,AA基因型个体的肌内脂肪(IMF)和血清总胆固醇(TC)显著高于BB基因型个体(P<0.05),而失水率则显著低于BB基因型个体(P<0.05)。结果揭示,A167G位点对脂肪沉积、失水率和胆固醇浓度可能有显著的遗传效应。 |
| 关 键 词： | 白羽番鸭 脂联素基因 PCR-SSCP 关联分析 |
Polymorphism in Exon-1 of Adiponectin Gene and Its Association with Meat Quality and TC and TG in White Muscovy |
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| ZHANG Yi-yu,XU Qi,DUAN Xiu-jun,ZHANG Hai-bo,ZHAO We-nming,SUN Guo-bo,CHEN Guo-hong.Polymorphism in Exon-1 of Adiponectin Gene and Its Association with Meat Quality and TC and TG in White Muscovy[J].Acta Agriculturae Universitis Jiangxiensis,2010,32(1). | |
| Authors: | ZHANG Yi-yu XU Qi DUAN Xiu-jun ZHANG Hai-bo ZHAO We-nming SUN Guo-bo CHEN Guo-hong |
| Institution: | 1.College of Animal Science and Technology/a>;Yangzhou University/a>;Yangzhou 225009/a>;China/a>;2.College of Animal Science/a>;Guizhou University/a>;Guiyang 550025/a>;3.Jiangsu Animal Husbandry & Veteri-nary College/a>;Taizhou 225300/a>;China |
| Abstract: | The 230 bp fragment of adiponectin gene exon 1 of White Muscovy was amplified with the primer designed from Cairina moschata DNA sequence(GQ873067),and its polymorphism was detected by PCR-SSCP method,and then its association with meat quality and serum total cholesterol(TC) and triglyceride(TG) were analyzed.2 alleles(A and B) and 3 genotypes(AA,AB and BB) were observed,in which A and AA were predominant.One SNP(A167G) was found in exon 1 of adiponectin gene,which belonged to silent mutation.The A167G loci... |
| Keywords: | White Muscovy adiponectin gene PCR-SSCP association analysis |
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