| 丙型肝炎病毒NS4B蛋白调控Hep3B细胞非折叠蛋白质反应研究 |
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| 引用本文： | 张艳妮,张庆华,陈瑶,刘好桔,刘志文,孔令保.丙型肝炎病毒NS4B蛋白调控Hep3B细胞非折叠蛋白质反应研究[J].江西农业大学学报,2012(1):165-169. |
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| 作者姓名： | 张艳妮 张庆华 陈瑶 刘好桔 刘志文 孔令保 |
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| 作者单位： | 江西农业大学生物科学与工程学院 |
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| 基金项目： | 江西省科技支撑计划重点项目(2009BNA09400);江西省自然科学基金项目(2010GZY0059);江西农业大学校基金项目(2950) |
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| 摘 要： | 研究表达的丙型肝炎病毒(HCV)NS4B对Hep3B细胞非折叠蛋白质反应的影响。NS4B重组真核表达质粒pcDNA3.1(-)NS4B通过脂质体转染Hep3B细胞,G418筛选和Western blot鉴定稳定转染细胞;RT-PCR检测稳定转染细胞内XBP1 mRNA剪接,Western blot鉴定ATF6蛋白切割,荧光素酶试验检测稳定转染细胞内GRP78和XBP1启动子活性。G418筛选和Western Blot鉴定证实获得稳定表达NS4B的Hep3B细胞;在该细胞内,XBP1 mRNA剪接、ATF6切割、XBP1和Grp78启动子激活均被检测到。NS4B在Hep3B的稳定表达诱导了非折叠蛋白质反应。
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| 关 键 词： | 丙型肝炎病毒 NS4B Hep3B 非折叠蛋白质反应 |
Regulation of Unfolded Protein Response in Hep3B Cells by HCV NS4B |
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| ZHANG Yan-ni,ZHANG Qing-hua,CHENG Yao, LIU Hao-ju,LIU Zhi-wen,KONG Ling-bao.Regulation of Unfolded Protein Response in Hep3B Cells by HCV NS4B[J].Acta Agriculturae Universitis Jiangxiensis,2012(1):165-169. |
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| Authors: | ZHANG Yan-ni ZHANG Qing-hua CHENG Yao LIU Hao-ju LIU Zhi-wen KONG Ling-bao |
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| Institution: | *(College of Bioscience and Engineering,JAU,Nanchang 330045,China) |
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| Abstract: | To study the effect of hepatitis C virus NS4B on unfolded protein response in Hep3B cells.The recombinant vector pcDNA3.1(-) NS4B with NS4B gene was transfected to Hep3B cells using Lipofectamine 2000.Stably transfected cells were selected by G418 and then confirmed by Western blot analysis;XBP1 mRNA splicing,ATF6 cleavage,GRP78 and XBP1 promoter activation were analyzed in stably transfected Hep3B cells using RT-PCR,Western blot or luciferase assays.G418 seclection and Western blot demonstrated that Hep3B cells stably expressing NS4B were obtained successfully;XBP1 mRNA splicing,ATF6 cleavage,GRP78 and XBP1 promoter activation were observed in Hep3B cells stably expressing NS4B.Stable expression of NS4B in Hep3B cells induces unfolded protein response. |
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| Keywords: | HCV NS4B Hep3B unfolded protein response |
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