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| 巴拉圭茶多酚大孔吸附树脂分离工艺及总多酚、多酚流分的抑菌作用研究 | |
| 引用本文： | 熊建华,刘仲华,黄建安,王克勤.巴拉圭茶多酚大孔吸附树脂分离工艺及总多酚、多酚流分的抑菌作用研究[J].江西农业大学学报,2007,29(4):690-694. |
| 作者姓名： | 熊建华 刘仲华 黄建安 王克勤 |
| 作者单位： | 1. 湖南农业大学,天然产物研究中心,湖南,长沙,410128;江西农业大学,食品科学系,江西,南昌,330045 2. 湖南农业大学,天然产物研究中心,湖南,长沙,410128 3. 湖南农业大学,天然产物研究中心,湖南,长沙,410128;湖南省原子能农业应用研究所,湖南,长沙,410125 |
| 基金项目： | 国家科技部科技型中小企业技术创新基金((2001)010418) |
| 摘 要： | 以静态吸附与解吸实验筛选树脂并研究巴拉圭茶多酚的大孔吸附树脂分离工艺及总多酚、多酚流分的抑菌作用。结果显示,HPD500是巴拉圭茶多酚良好的吸附剂,以水、φ=27%、φ=40%、φ=70%的乙醇为解吸剂的梯度洗脱,巴拉圭茶多酚被分离成为极性不同的3个流分。巴拉圭茶多酚具有较好的抑菌活性,其对沙门氏菌的抑菌活性强于茶多酚,不同极性的流分的抑菌作用弱于总多酚。 |
| 关 键 词： | 巴拉圭茶 多酚 大孔树脂 抑菌活性 |
| 文章编号： | 1000-2286（2007）04-0690-05 |
| 收稿时间： | 2007-04-17 |
| 修稿时间： | 2007-04-172007-06-04 |
A Study on Isolation Procedure of Ilex paraguariensis Polyphenols with Macroporou Resin and Antibacterial Activities of Total and Fractional Polyphenols |
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| XIONG Jian-hua,LIU Zhong-hua,HUANG Jian-an,WANG Ke-qin.A Study on Isolation Procedure of Ilex paraguariensis Polyphenols with Macroporou Resin and Antibacterial Activities of Total and Fractional Polyphenols[J].Acta Agriculturae Universitis Jiangxiensis,2007,29(4):690-694. | |
| Authors: | XIONG Jian-hua LIU Zhong-hua HUANG Jian-an WANG Ke-qin |
| Institution: | 1. Natural Products Research Center, HAU, Changsha 410128, China; 2. Department of Food Science, JAU,Nanchang 330045, China; 3. Hunan Institute of Atomic Energy Application in Agricuhure, Changsha 410125, China |
| Abstract: | In order to study separation of resin elution test,isolation procedure of Ilex paraguariensis polyphenols with macroporou resin and antibacterial activities of total and fractional polyphenols were studied.The results showed that HPD500 was optimal absorbent to isolate Ilex paraguariensis polyphenols.Three fractions with different polarity from IP polyphenols were obtained after gradient elution with aqueous alcohol.Ilex paraguariensis polyphenols showed considerable antibacterial effects and were more effective against solmonella paratyhpi than tea ployphenols.The antibacterial activity of the fractions were weaker than that of total ployphenols. |
| Keywords: | Ilex paraguariensis polyphenols macroporou resin antibacterial activities |
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