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| 茶树花药培养植株若干株系的RAPD分析 | |
| 引用本文： | 郭玉琼,赖钟雄,郭志雄,桑庆亮,赖呈纯,詹梓金,陈振光.茶树花药培养植株若干株系的RAPD分析[J].江西农业大学学报,2002,24(6):820-823. |
| 作者姓名： | 郭玉琼 赖钟雄 郭志雄 桑庆亮 赖呈纯 詹梓金 陈振光 |
| 作者单位： | 1. 福建农林大学亚热带果树研究所,福建福州,350002;福建农林大学园艺学院,福建福州,350002 2. 福建农林大学亚热带果树研究所,福建福州,350002 3. 福建农林大学园艺学院,福建福州,350002 |
| 基金项目： | 福建农林大学青年教师基金项目 (0 1B0 2 ) |
| 摘 要： | 对茶树花药培养植株 6个株系 (含母树 )进行RAPD分析 ,用 6条 10聚体的随机引物共得到 190个扩增位点 ,其中多态位点有 76个 ,占扩增出的总位点的 4 0 % ;在检测到的 4 4条谱带中 ,2 5条具有多态性 ,多态性程度达 5 6 .8% ,表明茶树花药培养植株各株系之间在DNA序列上存在着差异。进行聚类分析 ,将 6个株系划分为两个类群 ,并在DNA分子水平上进一步证实株系 4为花粉植株。 |
| 关 键 词： | 茶树 花药培养植株 RAPD |
| 文章编号： | 1000-2286(2002)06-0820-04 |
| 修稿时间： | 2002年9月20日 |
RAPD Analysis of Several Lines of Tea Plants from Anther Culture |
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| GUO Yu-qiong ,LAI Zhong-xiong ,GUO Zhi-xiong SANG Qing-liang ,LAI Cheng-chun ,ZHAN Zi-jin ,CHEN Zhen-guang.RAPD Analysis of Several Lines of Tea Plants from Anther Culture[J].Acta Agriculturae Universitis Jiangxiensis,2002,24(6):820-823. | |
| Authors: | GUO Yu-qiong LAI Zhong-xiong GUO Zhi-xiong SANG Qing-liang LAI Cheng-chun ZHAN Zi-jin CHEN Zhen-guang |
| Institution: | GUO Yu-qiong 1,2,LAI Zhong-xiong 1,GUO Zhi-xiong 2SANG Qing-liang 1,LAI Cheng-chun 1,ZHAN Zi-jin 2,CHEN Zhen-guang 1 |
| Abstract: | lines of tea (Camellia sinensis) anther plants(including the parent tree) were analyzed by RAPD technique. 190 sites were totally detected by 6 random 10-mer oligo-mucleotide primers, among which there were 76 polymorphic sites that occupied 40% of the whole amplied sites. Among the total 44 DNA bands, 25 bands were polymorphic, the polymorphic degree reached 56.8%. The results proved that the lines had high genetic diversity. These 6 tea lines were divided into 2 groups by UPGMA, moreover, the 4th line was proved to be pollen plant on DNA molecular level. |
| Keywords: | tea anther plant RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |