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| 枇杷主要种类的RAPD分析 | |
| 引用本文： | 陈义挺,赖钟雄,郭志雄,郭玉琼,蔡英卿,陈菁瑛,陆修闽,吴金寿.枇杷主要种类的RAPD分析[J].江西农业大学学报,2003,25(2):258-261. |
| 作者姓名： | 陈义挺 赖钟雄 郭志雄 郭玉琼 蔡英卿 陈菁瑛 陆修闽 吴金寿 |
| 作者单位： | 1. 福建农林大学,亚热带果树研究所,福建,福州,350002;福建省农业科学院,果树研究所,福建,福州,350013 2. 福建农林大学,亚热带果树研究所,福建,福州,350002 3. 福建省农业科学院,果树研究所,福建,福州,350013 |
| 基金项目： | 福建省政府重点建设学科基金，福建省农科院果树所所长基金项目 |
| 摘 要： | 应用14个10聚体随机引物对解放钟、森尾早生、旱钟6号、贵州野生、台湾枇杷、栋叶枇杷、乌脐、白梨、洛阳青、俨糖枇杷、湖北六二等11个枇杷品种或种类的基因组DNA进行RAPD扩增，共得到130条扩增带，其中33条为非多态性条带，多态性程度为74．62％。进行聚类分析，以D1＝0．599进行划分时，可将枇杷的11个遗传资源分成栽培和非栽培两个类群。以D2＝0．649进行划分时，8个普通种可分为两个类群，即白肉类群和红肉类群，并在DNA分子水平上说明枇杷果肉色泽可作为分类的一个指标。运用RAPD技术为枇杷遗传资源的鉴定和分类提供了新的途径。 |
| 关 键 词： | 枇杷 RAPD分析 品种 种类 遗传资源 |
| 文章编号： | 1000-2286(2003)02-0258-04 |
| 修稿时间： | 2002年11月18 |
RAPD Analysis of Main Species and Cultivars in Eriobotrya |
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| CHEN Yi-ting ,LAI Zhong-xiong ,GUO Zhi-xiong ,GUO Yu-qiong CAI Ying-qin ,CHEN Jing-ying ,LU Xiu-min ,WU Jin-shou.RAPD Analysis of Main Species and Cultivars in Eriobotrya[J].Acta Agriculturae Universitis Jiangxiensis,2003,25(2):258-261. | |
| Authors: | CHEN Yi-ting LAI Zhong-xiong GUO Zhi-xiong GUO Yu-qiong CAI Ying-qin CHEN Jing-ying LU Xiu-min WU Jin-shou |
| Institution: | CHEN Yi-ting 1,2,LAI Zhong-xiong 1,GUO Zhi-xiong 1,GUO Yu-qiong 1CAI Ying-qin 1,CHEN Jing-ying 2,LU Xiu-min 2,WU Jin-shou 1 |
| Abstract: | accessions of loquat genetic resources, including Eriobotrya japonica (cvs. Jiefangzhong, Moriowase, Zaozhong No.6, Wuqi, Baili, Luoyangqing, Yantangpipa, Hubeiliuer) E. prinoides, E. deflexa, Guizhouyesheng(wild loquat from Guizhou Province), were analyzed by RAPD with 14 arbitrary 10-mer primers. Total 130 DNA bands were amplified, among which 3 bands were common. The genetic diversity degree was 97.9%. By UPGMA (unweighted pair group with mathematic averages), given D1=0.599,11 loquat genetic germplasms were divided into 2 groups(cultivated group and un-cultivated group); given D2=0.649, E. japonica were divided into two groups (white-color and red-color pulp group). Moreover, the pulp color was proved to be a taxonomic index on DNA molecular level in loquat. RAPD technique has provided a new path for identification and classification of loquat genetic germplasm. |
| Keywords: | loquat RAPD genetic relationship genetic diversity |
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