培养基和植物激素对青钱柳茎段和叶片愈伤组织诱导的研究

为建立青钱柳组培快繁体系以解决青钱柳资源贫乏问题,以青钱柳茎段和叶片为外植体,对影响青钱柳愈伤组织诱导的基本培养基、激素种类及浓度等关键因素进行了研究。结果表明,基本培养基以改良MS诱导茎段愈伤组织效果最好,诱导率可达89.09%;MS诱导叶片愈伤组织效果最好,诱导率可达81.82%;2,4-D比NAA能够较快地诱导外植体产生愈伤组织,其中2.0 mg/L 2,4-D诱导率最高,可达88.57%,且形成愈伤组织多。2,4-D2.0 mg/L 6-BA1.0 mg/L的组合对青钱柳茎、叶诱导愈伤组织效果最佳,茎、叶诱导率分别可达91.57%和86.14%,且诱导出的愈伤组织生长快。

A Study on Effects of Media and Plant Hormones on Callus Induction of Cyclocarya paliurus (Batal.)Iljinskaja Stems and Leaves in Vitro

The effects of the basic media,the types and concentrations of plant hormones on callus induction of cyclocraya paliurus stems and leaves were studied in order to develop the Cyclocraya paliurus tissue culture system and resolve the problem of resouces scarceness.The results showed that improved MS medium was the best for stem callus induction,the induction rate was 89.09%;MS medium was the best for leaf callus induction,the induction rate was 81.82%;2,4-D was more effective in quickening callus induction than NAA,the medium of 2.0 mg/L got the highest induction rate which was 88.57%,and the explants formed more callus.The explants had the highest induction rate of callus on the medium of improved MS 2,4-D2.0mg/L 6-BA1.0mg/L,the induction rate of stems and leaves were 91.57% and 86.14%,and the calluses grew fast.