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| 内切酶接头引物对柿属SSAP分子标记扩增的影响 | |
| 引用本文： | 杜晓云,黄建民,张青林,罗正荣.内切酶接头引物对柿属SSAP分子标记扩增的影响[J].江西农业大学学报,2010,32(2). |
| 作者姓名： | 杜晓云 黄建民 张青林 罗正荣 |
| 作者单位： | 1. 江西省农业科学院,园艺研究所,江西,南昌,330200;华中农业大学,园艺植物生物学教育部重点实验室,湖北,武汉,430070 2. 江西省农业科学院,园艺研究所,江西,南昌,330200 3. 华中农业大学,园艺植物生物学教育部重点实验室,湖北,武汉,430070 |
| 基金项目： | 国家自然科学基金(30871686);;华中农业大学科研启动基金(20070504011);;江西省农业科学院创新基金(博士启动项目:2009博-8) |
| 摘 要： | SSAP(sequence-specificam plification polymorphism,序列特异扩增多态性)是种质遗传多样性分析和系统进化研究的有力工具之一。根据10个SSAP引物组合对28份柿属基因型的扩增结果探讨内切酶因素对SSAP逆转座子分子标记扩增的影响。结果表明,在以MseI和EcoRI组合消化的SSAP反应系统,且均以3个碱基作为末端选择性碱基的前提下,MseI与逆转座子引物组合的SSAP扩增性能优于EcoRI与逆转座子引物组合。研究结果为有目的和高效选择内切酶引物进行植物种质资源SSAP分析提供参考。 |
| 关 键 词： | 柿 逆转座子 SSAP分子标记 |
Influence of Restriction Endonuclease on the Performance of Retrotransposon-based SSAP Amplification in Diospyros spp. |
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| DU Xiao-yun,HUANG Jian-min,ZHANG Qing-lin,LUO Zheng-rong.Influence of Restriction Endonuclease on the Performance of Retrotransposon-based SSAP Amplification in Diospyros spp.[J].Acta Agriculturae Universitis Jiangxiensis,2010,32(2). | |
| Authors: | DU Xiao-yun HUANG Jian-min ZHANG Qing-lin LUO Zheng-rong |
| Institution: | 1.Institute of Horticultural Sciences/a>;Jiangxi Academy of Agricultural Sciences/a>;Nanchang 330200/a>;China/a>;2.Key Laboratory of Horticultural Plant Biology Affiliated to Ministry of Education/a>;Huazhong Agricultural University/a>;Wuhan 430070/a>;China |
| Abstract: | SSAP (Sequence-Specific Amplification Polymorphism) molecular marker has proven to be highly efficient in estimating genetic diversity and determining phylogenetic relationships in plant.Based on the result of SSAP molecular data derived from 10 primer combinations on 28 Diospyros spp.,the influence of restriction endonuclease on the performance of SSAP amplification was conducted.The results showed that when the SSAP analysis was digested by the combination of Mse I and EcoR I restriction endonuclease,more... |
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