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侧柏叶乙醇提取物抗氧化性的研究
引用本文:李雪,张建,徐瑞红,张鸣,高天鹏.侧柏叶乙醇提取物抗氧化性的研究[J].江西农业大学学报,2012(4):805-808.
作者姓名:李雪  张建  徐瑞红  张鸣  高天鹏
作者单位:兰州城市学院化学与环境科学学院;敦煌研究院文物保护技术服务中心
基金项目:甘肃省科技支撑计划(1011FKCA119)
摘    要:采用分光光度法测定不同质量浓度的侧柏叶乙醇提取物对羟基自由基、超氧自由基的清除能力及其还原能力。结果表明:随着侧柏叶乙醇提取物质量浓度的增加,还原能力、清除羟基自由基和超氧自由基能力都随着增强,所有提取物清除羟基自由基的活性均高于超氧自由基。此外在高实验质量浓度下,50%乙醇提取物清除羟基自由基的能力最强,还原能力和清除超氧自由基的能力由强到弱依次为50%乙醇提取物、95%乙醇提取物、70%乙醇提取物。

关 键 词:侧柏叶  乙醇提取物  抗氧化性

A Study on the Antioxidant Activity of the Ethanol Extract from Platycladus orientalis Leaves
LI Xue,ZHANG Jian,XU Rui-hong,ZHANG Ming,GAO Tian-peng.A Study on the Antioxidant Activity of the Ethanol Extract from Platycladus orientalis Leaves[J].Acta Agriculturae Universitis Jiangxiensis,2012(4):805-808.
Authors:LI Xue  ZHANG Jian  XU Rui-hong  ZHANG Ming  GAO Tian-peng
Institution:1(1.School of Chemistry and Environment Sciences,Lanzhou City University,Lanzhou 730070,China;2.Centre for Cultural Heritage Conservation Technology Services,Dunhuang Academy,Dunhuang 736200,China)
Abstract:Using the spectrophotometric method,the antioxidant activity of the extracts from Platycladus orientalis leaves under different concentrations of ethanol was studied by means of determining their reducing capacity,scavenging action to hydroxyl free radical(·OH) and superoxide anion free radical(·O2-).The results showed the reducing capacity,scavenging effects on hydroxyl free radical(·OH) and superoxide anion free radical(·O2-) were elevated with the concentration of ethanol extracts from Platycladus orientalis,and the effect of scavenging hydroxyl radicals was higher than that of superoxide anion radical.At high concentration,50% ethanol extract demonstrated the strongest scavenging action to hydroxyl free radical(·OH),the reducing capacity and scavenging action to superoxide anion free radical(·O2-) were in the order of 50% ethanol extract,95% ethanol extract,70% ethanol extract.
Keywords:Platycladus orientalis leaves  ethanol extract  antioxidant activity
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