| 苏云金芽胞杆菌cry1Ia17基因克隆、表达的研究 |
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| 引用本文： | 张庆丽,李海涛,赵勇,高继国.苏云金芽胞杆菌cry1Ia17基因克隆、表达的研究[J].东北农业大学学报,2011,42(4):93-97. |
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| 作者姓名： | 张庆丽 李海涛 赵勇 高继国 |
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| 作者单位： | 东北农业大学生命科学学院,哈尔滨,150030 |
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| 基金项目： | 转基因生物新品种培育重大专项，植物病虫害生物学国家重点实验室2010年度开放基金资助课题 |
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| 摘 要： | 根据cry1I类基因的全长序列设计引物,以苏云金芽胞杆菌(Bacillus thuringiensis,Bt)菌株BtMX2的总DNA为模板扩增出片段长为2.1 kb的cry1I的全长基因,插入大肠杆菌(Escherichia coli)表达栽体pEB,转化大肠杆菌Rosetta茵株,诱导表达出81 ku的蛋白,编码的...
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| 关 键 词： | 苏云金芽胞杆菌 cry1Ia基因 克隆 表达 |
Cloning, expression and activity of cry1/a17 gene from Bacillus thuringiensis isolate |
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| ZHANG Qingli,LI Haitao,ZHAO Yong,GAO Jiguo.Cloning, expression and activity of cry1/a17 gene from Bacillus thuringiensis isolate[J].Journal of Northeast Agricultural University,2011,42(4):93-97. |
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| Authors: | ZHANG Qingli LI Haitao ZHAO Yong GAO Jiguo |
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| Institution: | (College of Life Sciences,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | A full-length cry1Ia gene fragment,which obtained by PCR amplification with a pair of primers designed according to cry1I-type gene sequences and DNA from Bacillus thuringiensis BtMX2 as template,was introduced into expression vector pEB and transformed into Escherichia coli Rosetta,the predicted MW was 81 ku.Molecular weight of the induced express product was 81 ku.The encoded protein was composed of 720 amino acid residues,the isoelectric point of the protein was 6.09,and it was the weak acid protein.The amino acid sequence of cry1Ia17 was with an identity of 99% to cry1Ia9,the difference was two amino acids.The gene accession number was GU989199 and was designated as cry1Ia17 by International Nomenclature Committee of Bt. |
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| Keywords: | Bacillus thuringiensis cry1Ia gene cloning expression |
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