| 产蛋白酶和纤维素酶纳豆芽孢杆菌益生菌株的筛选及其生长特性研究 |
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| 引用本文： | 孙妍,王加启,奚晓琦,魏宏阳,周凌云.产蛋白酶和纤维素酶纳豆芽孢杆菌益生菌株的筛选及其生长特性研究[J].东北农业大学学报,2011,42(3):39-43. |
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| 作者姓名： | 孙妍 王加启 奚晓琦 魏宏阳 周凌云 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京,100193 |
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| 基金项目： | 动物营养学国家重点实验室资助研究课题(2004DA125184 |
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| 摘 要： | 根据纳豆芽孢杆菌(Bacillus subtilis natto)水解酪蛋白和羧甲基纤维素的生物学特性,以菌株NB-1和NR-l为出发菌株,采用稀释涂平板法获得10株初筛纳豆芽孢杆菌,通过测定初筛菌株48 h发酵液中蛋白酶活性和纤维素酶活性,确定NY-3产蛋白酶和纤维素酶活性均相对较高.同时对该菌株生长特性进行了研究....
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| 关 键 词： | 纳豆芽孢杆菌 蛋白酶 纤维素酶 筛选 生长特性 |
Screening of protease and cellulase producing Bacillus subtilis Natto strain and its growth characteristics |
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| SUN Yan,WANG Jiaqi,XI Xiaoqi,WEI Hongyang,ZHOU Lingyun.Screening of protease and cellulase producing Bacillus subtilis Natto strain and its growth characteristics[J].Journal of Northeast Agricultural University,2011,42(3):39-43. |
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| Authors: | SUN Yan WANG Jiaqi XI Xiaoqi WEI Hongyang ZHOU Lingyun |
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| Institution: | (State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
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| Abstract: | According to the biological qualification of hydrolysised casein and CMC of Bacillus subtilis Natto,10 strains were screened by pour plate method from their parent strains of Bacillus subtilis natto NB-1 and NR-1.The strain named NY-3 was selected with higher protease activity and cellulase activity through the test of protease activity and cellulase activity in 48 h fermenting medium among 10 strains.Further research of its growth characteristics showed that the best time of inoculation and fermentation were 14 and 48 h,respectively. |
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| Keywords: | Bacillus subtiliss Natto protease cellulose screening growth characteristic |
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