利用天然四倍体泥鳅生产全三倍体泥鳅的初步研究

2008～2009年利用从湖北省引进的天然四倍体(雄)泥鳅与当地的二倍体(雌)泥鳅杂交,进行全三倍体泥鳅培育的初步研究。结果表明,杂交组(2n×4n)平均受精率、孵化率、成活率分别为54.69%、80.64%、56.64%;对照组(2n×2n)分别为76.23%、82.97%、79.44%。早期胚胎染色体倍性鉴定结果表明,杂交组后代染色体数为71~75条,视为100%三倍体。6月龄时对其杂交后代进行流式细胞仪检测,均为100%三倍体。研究结果表明,利用湖北省洪湖市四倍体泥鳅与当地的二倍体泥鳅杂交能产生100%全三倍体泥鳅,这种生物杂交的方法操作简单、方法可靠,是生产全三倍体泥鳅的最佳途径。

Study on All-triploid production by crossing Misgurnus anguillicaudatus tetraploids and diploids

Nature tetraploid male loaches collected from Hubei Province were crossed with diploid female from Dalian,and all-triploid loach seeds were produced.The results showed that,the average fertilizing rate,hatching rate and survival rate in the cross group(2n ×4n) were 54.69%,80.64% and 56.64%,respectively,and those in the control group(2n ×2n) were 76.23%,82.97% and 79.44%,respectively.The chromosome number of the early embryos of the progeny was 71-75,which could be considered as 100% triploidy.The result of DNA-content flow cytometry on the 6 months old progeny was also conducted to confirm the ploidy status,showing 100% triploidy.The result of the present research showed that the cross between nature tetraploid loaches from Honghu,Hubei and nature diploid loaches from Dalian could produce 100% triploid loaches.This cross method was simple to operate,reliable,and the best way to produce all-triploid loaches.