复方中草药对镜鲤(Cyprinus carpio L.)血清转氨酶及红细胞抗氧化酶活性的影响

在基础日粮中添加2%由不同比例的刺五加、枸杞子、金银花和黄芪配伍的复方中草药饲喂镜鲤(Cyprinus carpio L).,于第14天、第28天及第42天检测红细胞的过氧化氢酶(CAT)、超氧化物岐化酶(SOD)、血清的谷草转氨酶(GOT)以及谷丙转氨酶(GPT)活性,以研究复方中草药对镜鲤的毒副作用。在28及42d时,复方Ⅰ组红细胞CAT活性与对照组差异显著(P0.05);28d时,各复方组红细胞SOD活性均与对照组差异极显著(P0.01);28d时,复方Ⅱ组血清GOT活性及复方Ⅲ组血清GPT活性与对照组差异极显著(P0.05);42d时,各复方组血清GPT活性与对照组差异极显著(P0.01)。结果表明,试验复方中药添加到镜鲤的饲料中,可以增强其血清转氨酶及红细胞抗氧化酶活性,其中复方Ⅰ效果最佳,建议用药时间为28d。

Effect of Chinese herb compounds on activities of transaminase in-serum and antioxidase in erythrocyte of Cyprinus carpio L.

The researchwas carriedontoxicityofChinese herbcompounds making to Cyprinus carpio L., they were fed with basical formulated diet supplemented seperated with 2% compound Chinese herbals (Acanthopanax senticosus Harms, L. chinense Mill, Lonicera japonica Thunb, Astragalus membranaceu). The activities of CAT and SOD in erythrocyte and activities of GOT and GPT serum were measured respectively on the 14th, 28th and 42th days. The results showed that the CAT activities in erythrocyte of compoundⅠhad significant difference with the control group(P<0.05). When on the 28th and 42th days, only compoundⅠimproved activities of CAT in erythrocyte, the othercompands did not have the singicant difference compared to the control group. On the 28th day, SOD activities in erythrocyte of all compounds had very significant difference with the control group (P<0.01). On the 28th day, GOT activities in serum of compoundⅡ and compoundⅢ had very significant difference with the control group (P<0.05), the other compand groups didn't have signicant difference with the control group; on the 42th day, GPT activities in serum ofallcompounds had very signicantdifference with the controlgroup(P<0.01). The results showed that Chinese herb compounds could be added to feed in Cyprinus carpio L. to increase its serum transaminaseand erythrocyte antioxidant enzyme activities, which was suggested that the medicated time was 28 d, and the mosteffective compound was compound .