| 大豆原生质体培养基的研究 |
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| 引用本文： | 张贤泽.大豆原生质体培养基的研究[J].东北农业大学学报,1992(4). |
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| 作者姓名： | 张贤泽 |
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| 作者单位： | 东北农学院生物工程系 哈尔滨150030 |
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| 摘 要： | 本试验制成大豆未成熟子叶原生质体再生细胞分裂率高、能保持胚性状态并最终能分化成正常植株的培养基。试验发现,天冬酰胺、谷氨酰胺、尿囊素和尿囊酸等大豆根瘤形成的酰尿有机态氮是有效的主要氮源。
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| 关 键 词： | 大豆 原生质体 培养基 胚性愈伤组织 尿囊素 尿囊酸 植株再生 |
STUDIES ON THE MEDIA OF SOYBEAN PROTOPLAST CULTURE |
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| Zhang Xianze.STUDIES ON THE MEDIA OF SOYBEAN PROTOPLAST CULTURE[J].Journal of Northeast Agricultural University,1992(4). |
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| Authors: | Zhang Xianze |
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| Abstract: | The studies indicated that asparagine,glutamine,allantoin,and allantoic acid—ureide
organic nitrogen formed by soybean root nodules—were the major effective nitrogen sources
in successful soybean protoplast culture.Soybean regenerated plants were obtained in the
studies when the media containing organic nitrogen sources mentioned above were used. |
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| Keywords: | soybean protoplast medium embryogenic callus allantoin allantoic acid plant regeneration |
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