| 虎尾兰的组织培养技术研究 |
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| 引用本文： | 黄凤兰,李静,张茜,张群,赵玲玲,聂民利.虎尾兰的组织培养技术研究[J].东北农业大学学报,2012(1):131-136. |
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| 作者姓名： | 黄凤兰 李静 张茜 张群 赵玲玲 聂民利 |
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| 作者单位： | 东北林业大学生命科学学院;内蒙古民族大学生命科学学院 |
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| 基金项目： | 内蒙古民族大学高教研究项目(2009053) |
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| 摘 要： | 虎尾兰(Sansevieria trifasciatavar.harnii)是龙舌兰科虎尾兰属植物,不但具有观赏价值,还有较高的营养价值,并且有净化空气的功效。为了在短时间内获得大量的虎尾兰植株,以短叶虎尾兰的叶片为外植体进行组织培养试验,采用组织培养育苗方法,建立从愈伤组织到再生植株的培养体系。结果表明,基本培养基为MS;诱导愈伤的最佳PGR浓度配比为1 mg·L-16-BA+0.5 mg·L-1NAA+0.5 mg·L-12,4-D;诱导芽的最佳PGR浓度配比为1 mg·L-16-BA+0.8 mg·L-1NAA;诱导生根的最佳PGR浓度为0.1 mg·L-1NAA。
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| 关 键 词： | 虎尾兰 组织培养 愈伤组织 |
Study on tissue culture technique of Sansevieria trifasciata var.harnii |
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| HUANG Fenglan,LI Jing,ZHANG Xi,ZHANG Qun,ZHAO Lingling,NIE Minli.Study on tissue culture technique of Sansevieria trifasciata var.harnii[J].Journal of Northeast Agricultural University,2012(1):131-136. |
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| Authors: | HUANG Fenglan LI Jing ZHANG Xi ZHANG Qun ZHAO Lingling NIE Minli |
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| Institution: | 1.College of Life Sciences,Northeast Forestry University,Harbin 150040,China;2.College of Life Sciences,Inner Mongolia University for the Nationality,Tongliao Inner Mongolia 028000,China) |
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| Abstract: | Sansevieria trifasciata var.harnii belongs to Agavaceae Sansevieria plant,which has view and nutritibe value,and being purifying the air.In order to obtain a lot of plants in short time,using leaves as explants and established regeneration system from callus.The results showed that MS was the basic medium;the optimum callus induction PGR was 1 mg·L-1 6-BA,0.5 mg·L-1 NAA and 0.5 mg·L-1 2,4-D;the optimum bud induction PGR was 1 mg·L-1 6-BA and 0.8 mg·L-1 NAA;and the optimal PGR induce rooting was 0.1 mg·L-1 NAA. |
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| Keywords: | Sansevieria trifasciata var harnii tissue culture callus |
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