马铃薯花药培养影响因素的研究

采用克新13号、克9723-20和讷16等8份马铃薯品种(系)为试验材料,采用L3(2 81×48)正交设计,对影响马铃薯花药褐化、愈伤组织诱导率和胚状体发生率等因素进行了系统研究。结果表明,马铃薯花药培养对基因型有很大的依赖性,讷16、克9723-20、俄8和波C等4份材料具有较强的花药培养再生能力;花药接种密度对褐化率影响较大,40个.瓶-1的花药接种密度比较合适;硝酸银浓度30 mg.L-1、活性炭浓度200 mg.L-1能有效地减轻花药的褐化程度。植物生长调节剂对愈伤组织诱导影响最大,NAA 0.5 mg.L-1、2,4-D 1.0 mg.L-1、KT 0.5 mg.L-1的配比适合多数材料愈伤组织的形成和胚状体的发生,以麦芽糖代替蔗糖可以提高愈伤组织的诱导率;培养基中加入马铃薯提取液能够促进胚状体的发生。

Effects of several factors on the anther culture of potato

Eight genotypes of potato were used to study the effects of brown anthers,cell yields and embryo induction rate through the orthogonal array of L32(81×48).The results showed that genotype was the major factor playing important role on potato anther culture.The regeneration abilities of the culfivars Ne16,Boc E8 and Ke 9723-20 are better than the others.The frequency of brown anther was affected by the density of pollens and the appropriate density was 40 per bottle.Media added 30 mg·L-1 AgNO3 and 0.4% active charcoal effectively reduced the brown tissues.It was optimal to induce the callus in MS with 0.5 mg·L-1 NAA,1.0 mg·L-1 2,4-D and 0.5 mg·L-1 KT.As a carbon source,maltose was more effective than sucrose on the callus induction;media added potato extract increased the embryo induction.