| 大鳞副泥鳅(2n♀)×泥鳅(4n♂)杂种后代染色体带型及FISH分析 |
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| 引用本文： | 贾光风,李雅娟,钱聪,高敏.大鳞副泥鳅(2n♀)×泥鳅(4n♂)杂种后代染色体带型及FISH分析[J].东北农业大学学报,2011,42(9):72-75,146. |
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| 作者姓名： | 贾光风 李雅娟 钱聪 高敏 |
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| 作者单位： | 1. 大连市甘井子区农林水利和海洋渔业局中国海监甘井子区大队,辽宁大连,116033
2. 大连海洋大学生命科学与技术学院,辽宁大连,116023 |
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| 基金项目： | 辽宁省科学技术计划重大、重点项目(2008203001) |
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| 摘 要： | 文章对二倍体大鳞副泥鳅♀×四倍体泥鳅♂杂交后代的染色体进行Ag-NORs、CMA3/DA/DAPI及FISH等系列研究.结果表明,①在对照组泥鳅×泥鳅(2n×2n)杂交后代的间期核和中期分裂相中最高银染点数为2个,杂交组2n×4n杂种后代的间期核和中期分裂相中最高银染点数为3个；②在对照组2n×2n杂交后代有丝分裂中期...
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| 关 键 词： | 大鳞副泥鳅 泥鳅 四倍体 Ag-NORs CMA3/DA/DAPI FISH |
Chromosome banding and FISH with rDNA probe in Paramisgumus dabryanus(2n ♀ ) × Misgurnus anguillicaudatus(4n ♂ ) |
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| JIA Guangfeng,LI Yajuan,QIAN Cong,GAO Min.Chromosome banding and FISH with rDNA probe in Paramisgumus dabryanus(2n ♀ ) × Misgurnus anguillicaudatus(4n ♂ )[J].Journal of Northeast Agricultural University,2011,42(9):72-75,146. |
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| Authors: | JIA Guangfeng LI Yajuan QIAN Cong GAO Min |
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| Institution: | JIA Guangfeng1,LI Yajuan2,QIAN Cong2,GAO Min2(1.China Marine Surveillance Corp of Ganjingzi District,Agriculture and Forestry Irrigation Bureau,Ganjingzi District Dalian,Dalian Liaoning 116033,China,2.Life Science and Technology Institute,Dalian Ocean University,Dalian Liaoning 116023,China) |
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| Abstract: | Chromosomes of breedings of the diploid Paramisgurnus dabryanus and tetraploid Misgurnus anguillicaudatus were analyzed by staining with Ag,chromomycinA3(CMA3)/ distamycin A(DA),and DA/4’,6-diamidino-2-phenylindole(DAPI),and using fluorescence in stu hybridization(FISH) with 5.8S+28SrDNA as a probe.Results were as follows:(1)The maximum number of Ag-NORs were two in both interphase and metaphase in the control group 2n×2n,while they were both three in the cross 2n×4n;(2)CMA3 positive sites were two in 2n×2n,on the telomeric position of the short arms of M chromosomes,while four positive sites in 2n×4n were detected on the same location.(3) By FISH,5.8S+ 28SrDNA signals were clearly detected in the above telomeric Ag-NOR and CMA3-positive sites in the short arms of M chromosome pair or trio.Two signals were detected in the control group,and three in the cross group.FISH position of ribosomal 5.8S+28S rDNA in hybrid progenies was corresponding with the results of Ag-NORs,CMA3/DA/DAPI,and the number were all three.Results provided an cytogenetic proof that the hybrid progenies were triploid. |
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| Keywords: | Paramisgurnus dabryanus Misgurnus anguillicaudatus tetraploids Ag-NORs CMA3/DA/DAPI FISH |
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