| 基于双单倍体牙鲆挖掘与生长性状连锁的微卫星标记 |
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| 引用本文： | 刘永新,王桂兴,王玉芬,司飞,孙朝徽,张晓彦,刘海金.基于双单倍体牙鲆挖掘与生长性状连锁的微卫星标记[J].东北农业大学学报,2011,42(9):104-110. |
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| 作者姓名： | 刘永新 王桂兴 王玉芬 司飞 孙朝徽 张晓彦 刘海金 |
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| 作者单位： | 1. 中国水产科学研究院,北京,100141
2. 中国水产科学研究院北戴河中心实验站,山东秦皇岛,066100 |
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| 基金项目： | 中国水产科学研究院院部基本科研业务费专项资金项目(2010C018); 国家现代产业技术体系资助项目(nycytx-50-G02); 公益性行业(农业)科研专项经费(200903046-07) |
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| 摘 要： | 利用中国水产科学研究院北戴河中心实验站2007年制备的双单倍体牙鲆为材料,根据生长性状表型值分成快速生长组(Fast growth group,FGG)和慢速生长组(Slow growth group,SGG),进行微卫星标记与生长性状的连锁分析.从牙鲆遗传连锁图谱选择40个高多态性的微卫星标记,覆盖了24个连锁群中的...
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| 关 键 词： | 双单倍体 牙鲆 微卫星 生长性状 |
Development of microsatellite markers correlation to growth traits based on double haploids of Japanese flounder |
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| LIU Yongxin,WANG Guixing,WANG Yufen,SI Fei,SUN Zhaohui,ZHANG Xiaoyan,LlU Haijin.Development of microsatellite markers correlation to growth traits based on double haploids of Japanese flounder[J].Journal of Northeast Agricultural University,2011,42(9):104-110. |
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| Authors: | LIU Yongxin WANG Guixing WANG Yufen SI Fei SUN Zhaohui ZHANG Xiaoyan LlU Haijin |
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| Institution: | LIU Yongxin1,WANG Guixing2,WANG Yufen2,SI Fei2,SUN Zhaohui2,ZHANG Xiaoyan2,LIU Haijin1(1.Chinese Academy of Fishery Sciences,Beijing 100141,China,2.Beidaihe Central Experiment Station,Chinese Academy of Fishery Sciences,Qinhuangdao Shandong 066100,China) |
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| Abstract: | The double haploids of Japanese flounder produced by Beidaihe Central Experiment Station of Chinese Academy of Fishery Sciences were divided into fast growth group(FGG) and slow growth group(SGG) according to phenotype value of growth traits,which materials were used to carry out linkage analysis between microsatellite markers and growth traits.Twenty-two out of 24 linkage groups were covered by 40 polymorphic microsatellite loci selected from the linkage map.PCR amplification on 30 individuals from FGG and... |
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| Keywords: | double haploid Japanese flounder microsatellite marker growth trait |
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