| 民猪群体氟烷基因频率检测及序列多态性分析 |
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| 引用本文： | 魏巍,王希彪,黄宣凯,张野,兰晓明,刘建明,暴国.民猪群体氟烷基因频率检测及序列多态性分析[J].东北农业大学学报,2011,42(9):31-36. |
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| 作者姓名： | 魏巍 王希彪 黄宣凯 张野 兰晓明 刘建明 暴国 |
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| 作者单位： | 东北农业大学动物科学技术学院,哈尔滨,150030 |
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| 基金项目： | 公益性行业科研专项(nyhyzx07-034); 国家科技支撑计划(2006BAD01A08-08;2008BADB2B02-05) |
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| 摘 要： | 重新确定现有民猪(原称东北民猪)群体中氟烷基因(Haln)的基因型,采用PCR-RFLP方法对48头民猪进行了氟烷(Haln)基因检测,并测定了其中5头民猪的Haln基因序列.结果表明,48头民猪中HalNHalN(NN)型占70.83％,HalNHaln(Nn)型占25％,HalnHaln(nn)型占4.17％；N基...
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| 关 键 词： | 民猪 Haln基因 PCR-RFLP 基因频率 多态性分析 |
Frequency detection and sequence polymorphism analyses of Hal~n gene in Min pig breed populations |
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| WEI Wei,WANG Xibiao,HUANG Xuankai,ZHANG Ye,LAN Xiaoming,LIU Jianming,BAO Guo.Frequency detection and sequence polymorphism analyses of Hal~n gene in Min pig breed populations[J].Journal of Northeast Agricultural University,2011,42(9):31-36. |
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| Authors: | WEI Wei WANG Xibiao HUANG Xuankai ZHANG Ye LAN Xiaoming LIU Jianming BAO Guo |
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| Institution: | WEI Wei,WANG Xibiao,HUANG Xuankai,ZHANG Ye,LAN Xiaoming,LIU Jianming,BAO Guo(College of Animal Sciences and Technology,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | To make sure halothane gene genetype of Minpig(known as Northeast Minpig),amplification the halothane gene of 48 Minpigs by PCR-RFLP,and sequencing five complete sequences of them.It provided theoretical basis of varietal improvement for Minpig protect populations.Found in Minpig HalNHalN(NN) type was 70.83%,HalNHaln(Nn) type was 25%,and HalnHaln(nn) type was 4.17%;N gene frequency was 83.33% and n gene frequency was 16.67%.Minpig halothane gene sequences had 99% homology with wild boar halothane gene seque... |
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| Keywords: | Min pig halothane gene PCR-RFLP gene frequency polymorphism analysis |
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