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| 齐墩果酸分光光度法测定大豆异黄酮粉中总皂苷含量 | |
| 引用本文： | 李成刚,张永忠,迟玉杰,付红.齐墩果酸分光光度法测定大豆异黄酮粉中总皂苷含量[J].东北农业大学学报,2008,39(7). |
| 作者姓名： | 李成刚 张永忠 迟玉杰 付红 |
| 作者单位： | 1. 东北农业大学理学院,哈尔滨,150030 2. 东北农业大学理学院,哈尔滨,150030;大豆生物学教育部重点实验室,哈尔滨,150030 3. 大豆生物学教育部重点实验室,哈尔滨,150030 |
| 基金项目： | 教育部重点实验室基金，黑龙江省科技攻关项目 |
| 摘 要： | 以大豆皂苷类似物齐墩果酸为标准品,经香草醛-高氯酸显色,应用可见分光光度法在560 nm处分别测定了20%、30%和40%大豆异黄酮粉中总皂苷的含量。测定结果表明,大豆总皂苷的对应含量分别为36.0%、26.3%、16.1%,该方法的线性范围为1～20μg.mL-1,相对标准偏差(RSD)为3.1%,平均加样回收率为101.5%,相关系数R2=0.9996。 |
| 关 键 词： | 大豆异黄酮 大豆皂苷 齐墩果酸 可见分光光度法 |
Determination of saponin in soybean isoflavone by oleanolic acid spectrotometry |
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| LI Chenggang,ZHANG Yongzhong,CHI Yujie,FU Hong.Determination of saponin in soybean isoflavone by oleanolic acid spectrotometry[J].Journal of Northeast Agricultural University,2008,39(7). | |
| Authors: | LI Chenggang ZHANG Yongzhong CHI Yujie FU Hong |
| Abstract: | In this paper,the oleanolic acid was taken as the reference standard using the method HClO4-vanillin colorimetry.The content of saponin in soybean isoflavone was determinated by spectrophotometry at 560 nm.The results showed that contents of soyasaponin were 36.0%,26.3% and 16.1%,respectively.The linear relation was 1-20 μg·mL-1,the RSD was 3.1%,the average recovery rate was 101.5%,and the coefficient correla-tion was 0.9996. |
| Keywords: | soybean isoflavone soyasaponin oleanolic acid spectrophotometry |
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