| 抗真菌转基因大豆对大豆疫霉根腐病抗病性鉴定 |
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| 引用本文： | 郭玉双,张艳菊,朱延明,刘佳,李杰,柏锡,张淑珍.抗真菌转基因大豆对大豆疫霉根腐病抗病性鉴定[J].东北农业大学学报,2006,37(6):733-738. |
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| 作者姓名： | 郭玉双 张艳菊 朱延明 刘佳 李杰 柏锡 张淑珍 |
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| 作者单位： | 1. 东北农业大学生命科学学院,黑龙江,哈尔滨,150030
2. 东北农业大学农学院,黑龙江,哈尔滨,150030 |
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| 基金项目： | 国家转基因植物专项基金 |
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| 摘 要： | 采用下胚轴伤口接种法,对Southern杂交阳性的转菜豆几丁质酶基因和大麦核糖体失活蛋白基因的双价转基因大豆T2代的5个株系,进行了大豆疫霉根腐病抗病性检测,并通过比较接种后转基因大豆与对照组的死亡率来探讨两者的抗病性差异。结果表明,有4个转基因株系对大豆疫霉根腐病的抗性与非转基因对照组相比有明显提高,另外1个株系与对照组相比没有明显差异。
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| 关 键 词： | 转基因大豆 菜豆几丁质酶基因 大麦核糖体失活蛋白基因 大豆疫霉根腐病 抗病性鉴定 |
| 文章编号： | 1005-9369（2006）06-0733-06 |
| 收稿时间： | 2005-09-07 |
| 修稿时间： | 2005年9月7日 |
Resistance identification of fungi-resistant genes transformed soybean to Phytophthora sojae |
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| GUO Yushuang,ZHANG Yanju,ZHU Yanming,LIU Jia,LI Jie,BAI Xi,ZHANG Shuzhen.Resistance identification of fungi-resistant genes transformed soybean to Phytophthora sojae[J].Journal of Northeast Agricultural University,2006,37(6):733-738. |
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| Authors: | GUO Yushuang ZHANG Yanju ZHU Yanming LIU Jia LI Jie BAI Xi ZHANG Shuzhen |
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| Institution: | 1. College of Life Sciences, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC; 2. College of Agriculture, Northeast Agricultural University, Harbin Heilongjiang 150086, PRC |
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| Abstract: | The resistance to Phytophthora sojae of 5 bivalent transgenic soybean lines was identified using the hypocotyls inoculation technique. The lines were the T2 of the transgenic soybean which were transformed with kidney bean chitinase gene and barley ribosome inactivating protein gene, and were positive by Southern blot analysis. The resistance difference was studied through comparing the death percentage of transgenic soybean with the control. The results showed that 4 lines were more resistant to Phytophthora sojae, whereas other one had no evident difference compared to the control. |
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| Keywords: | transgenic soybean kidney bean chitinase gene barley ribosome inactivating protein gene soybean Phytophthora sojae resistance identification |
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