| SYBR Green Ⅰ荧光检测痕量宿主DNA |
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| 引用本文： | 蔺芳,付瑞敏,皮川真,时凯,叶刚,江景玉,陈慧珍.SYBR Green Ⅰ荧光检测痕量宿主DNA[J].东北农业大学学报,2011(7):125-129. |
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| 作者姓名： | 蔺芳 付瑞敏 皮川真 时凯 叶刚 江景玉 陈慧珍 |
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| 作者单位： | 新乡学院生命科学与技术系;河南教育学院人口与生命科学系;邦和药业股份有限公司 |
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| 基金项目： | 河南教育学院青年科研课题(20100103) |
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| 摘 要： | 基于SYBR Green Ⅰ荧光染料与双链DNA结合产生荧光的原理,建立一种痕量DNA荧光检测方法.采用不同稀释倍数的SYBR Green Ⅰ荧光染料做DNA的标准曲线,确定SYBR Green Ⅰ荧光染料的最佳稀释倍数.比较荧光法和Southern blotting杂交法定量DNA.结果表明,SYBR Green Ⅰ...
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| 关 键 词： | 痕量DNA SYBR Green Ⅰ 荧光法 Southern印迹杂交 |
Detecting trace host DNA based on SYBR Green I fluorescence method |
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| LIN Fang,FU Ruimin,PI Chuanzhen,SHI Kai,YE Gang,JIANG Jingyu,CHEN Huizhen.Detecting trace host DNA based on SYBR Green I fluorescence method[J].Journal of Northeast Agricultural University,2011(7):125-129. |
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| Authors: | LIN Fang FU Ruimin PI Chuanzhen SHI Kai YE Gang JIANG Jingyu CHEN Huizhen |
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| Institution: | 1.Department of Life Science and Biotechnology,Xinxiang College,Xinxiang He’nan 453000,China;2.Population and Life Department,Henan Institute of Education,Zhengzhou 450046,China;3.Banghe Pharmaceutical Co.Ltd,Zhengzhou 450001,China) |
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| Abstract: | A fluorescence method for trace amount DNA detection was established based on the principle that combination of SYBR Green I fluorescent dyes and double-stranded DNA will generate fluorescent.Using different dilution of SYBR Green I fluorescent dye to do DNA standard curve to confirm the optimization of dilution for the SYBR Green I fluorescence dye.Comparison of detection results of quantitative DNA analysis between fluorescence and Southern blotting hybridization.The results showed that it could obtain the wide linear range and better linear relationship(R2=0.9999) when the dilution of SYBR Green I fluorescent dye was 1∶10 000,and the methods of fluorescence hybridization and Southern blotting could permit each other.SYBR Green I fluorescent analysis of quantitative DNA is a fast and stable detection method of trace DNA. |
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| Keywords: | trace DNA SYBR Green I fluorescence method Southern blotting |
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