两种致病菌对杂交东方鲀非特异性免疫和抗氧化指标影响

为研究迟钝爱德华氏菌和哈氏弧菌感染对杂交东方鲀"冀研一号"(菊黄东方鲀♀×红鳍东方鲀♂)非特异性免疫和抗氧化指标影响,分别对杂交东方鲀腹腔注射PBS、迟钝爱德华氏菌和哈氏弧菌,注射后6、12、18、24和36h分别取肝胰脏,测定其谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)、总超氧化物歧化酶(T-SOD)、谷草转氨酶(GOT)、谷丙转氨酶(GPT)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活力。结果表明,在攻毒后36 h内,注射迟钝爱德华氏菌"冀研一号"东方鲀肝胰脏总超氧化物歧化酶、碱性磷酸酶和酸性磷酸酶酶活均极显著升高(P0.01);攻毒后36 h内谷胱甘肽过氧化物酶、谷胱甘肽均表现升高趋势。感染哈氏弧菌后,肝胰脏中谷胱甘肽过氧化物酶、总超氧化物歧化酶活力均高于对照组;谷胱甘肽含量表现升高趋势。试验鱼体感染致病菌后,引起肝胰脏非特异性免疫和抗氧化指标变化。

Effect of nonspecific immune response and anti-oxidation of hybrid Puffer infected by two pathogens

To study the effect of Edwardsiella tarda and Vibrio harveyi challenge on the nonspecific immune function and anti-oxidation of hybrid Puffer,intraperitoneal injection PBS,Edwardsiella tarda and Vibrio harveyi,respectively.The hepatopancreas were taken at the 6,12,18,24 and 36 h after infection and the activities of GSH,GSH-PX,T-SOD,GOT,GPT,ACP,AKP enzymes were measured to study the variation of enzyme activities.The results showed that during the 36 h,the value of T-SOD,AKP,ACP increased (P＜0.01) in hepatopancreas,the value of GSH-PX and GSH increased in the same.After injection with Vibrio harveyi,the value of GSH-PX,T-SOD were higher than the control and GSH was increased in hepatopancreas.In this experiment,no matter what kind of fish pathogen infection,will cause changes of nonspecific immunity and antioxidant indices in the hepatopancreas.