水稻叶绿素含量的动态QTL剖析

为剖析水稻叶绿素不同时期的发育动态规律及其遗传机制,以沈农265和丽江新团黑谷的粳-粳交重组自交系为材料,对水稻分蘖期、抽穗期和成熟期的叶绿素含量进行动态QTL分析.共检测22个条件QTL和14个非条件 QTL,分布在第5条染色体以外的11条染色体上.控制分蘖期、抽穗期和成熟期叶绿素含量的条件QTL分别有5个、7个和10个;控制分蘖期-抽穗期和抽穗期-成熟期叶绿素含量的非条件QTL各有7个.进一步分析表明,在叶绿素含量动态发育的不同阶段,控制叶绿素含量QTLs的数目、效应及作用方式不同,反映出叶绿素生物合成过程的复杂性.与其他研究比较发现,定位在第1染色体 RM428-RM580区段、第3染色体RM426-RM514区段、第4染色体RM470-RM559区段、第8染色体RM408-RM25区段和第9染色体RM566-RM242区段的位点可以在不同群体和不同环境下稳定表达.其中,第3染色体上 qCT3a、qCH3、qCM3以及第9染色体qCT9、qCH9b和qCH9等区域是提高叶绿素含量的重要功能区.对这些区域开发稳定并易于检测的分子标记,可用于培育高产新品种

Dynamic QTL analysis on chlorophyll contents in rice

To understand the development patterns of chlorophyll content at different stages,the QTLs controlling chlorophyll content was analyzed at the stages of tillering,heading and maturity by employing a recombinant inbred lines(RILs) derived from a cross between two japonica rice cultivars,Shennong265 and Lijiangxintuanheigu.Detected total 22 and 14 QTLs distributing on all the rice chromosomes except chromosome 5.Five,seven and 10 QTLs controlling chlorophyll contents at tillering stage,heading stage and maturity stage were detected,respectively.QTLs controlling chlorophyll contents in tillering to heading stage and heading to maturity stage were all seven.Further research indicated that the numbers,effects and the operation manner of the QTLs affecting chlorophyll contents were different at different stages and demonstrated the complexity of biosynthesis.Comparison with other studies revealed that major QTLs were easily re-detected in different populations and environments.These QTLs located in RM428-RM580 of chromosome 1,RM426-RM514 of chromosome 3,RM470-RM559 of chromosome 4,RM408-RM25 of chromosome 8 and RM566-RM242 of chromosome 9.qCT3a,qCH3 and qCM3 on chromosome 3 and qCT9,qCH9b and qCH9 on chromosome 9 were important functional regions to improve chlorophyll content.The stable and easily detected molecular marker would be developed to breed new high yield varieties.