| 热激诱导番茄果实细胞色素C的释放 |
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| 引用本文： | 刘翔,杨明瑜,曹灿,姚丹,朱文华,喻实,曲桂芹.热激诱导番茄果实细胞色素C的释放[J].东北农业大学学报,2009,40(1). |
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| 作者姓名： | 刘翔 杨明瑜 曹灿 姚丹 朱文华 喻实 曲桂芹 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 摘 要： | 细胞程序化死亡(Programmed Cell Death,PCD)在植物生长发育及胁迫反应过程中起着重要的作用。线粒体中细胞色素C的释放是植物发生PCD的早期特征之一。番茄果实作为一种模式植物在热胁迫过程中有许多抗逆反应。试验优化了番茄果实线粒体蛋白和细胞质蛋白的提取及Western杂交检测技术,并检测到番茄果实经热激处理后细胞色素C从线粒体释放到细胞质,为番茄果实PCD的深入研究奠定基础。
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| 关 键 词： | 番茄果实 热激 细胞色素C |
Release of cytochrome C in tomato fruit induced by heat shock |
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| LIU Xiang,YANG Mingyu,CAO Can,YAO Dan,ZHU Wenhua,YU Shi,QU Guiqin.Release of cytochrome C in tomato fruit induced by heat shock[J].Journal of Northeast Agricultural University,2009,40(1). |
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| Authors: | LIU Xiang YANG Mingyu CAO Can YAO Dan ZHU Wenhua YU Shi QU Guiqin |
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| Institution: | College of Food Science and Nutritional Engineering;China Agricultural University;Beijing 100083;China |
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| Abstract: | Programmed cell death (PCD) plays an important role during the development and the stress response in plant. Release of Cytochrome C in the mitochondria is one of the inchoate diagnostic features in plant PCD. Tomato as a model material made lots of reverse reaction in stress response. This study optimized the extraction of mitochondrial and cytosol protein fraction from tomato fruit and western hybridization technique. Meanwhile, it showed that release of Cytochrome C which occured in tomato fruit after he... |
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| Keywords: | tomato fruit heat shock cytochrome C |
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