大豆粒形性状与百粒重的QTL定位

文章选用东农46和L-100杂交构建的F2:10、F2:11代大豆重组自交系群体127个家系为作图群体,通过全基因组重测序技术,开发bin标记,构建高密度遗传连锁图谱,结合两年四点的大豆粒形性状和百粒重表型数据,利用IciMapping 4.0软件的完备区间作图法作加性QTLs和QTLs间上位性互作检测。结果表明,经粒形性状和百粒重主效QTLs检测,获得81个与大豆粒长、粒宽、粒厚、粒体积和百粒重相关QTLs,分布于18条染色体,贡献率1.66%～30.70%,其中贡献率最高位点分别为qSL-4-2(23.85%)、qSW-1-1(15.40%)、qST-1-2(17.66%)、qSV-15-1(30.70%)和q100-SW-19-1(15.43%);经相关性状加性×加性上位性互作检测,获得43对大豆粒形性状和百粒重加性×加性上位互作效应QTLs,贡献率1.41%～23.19%。

Mapping QTL for seed shape traits and 100-seed weight in soybean

In this study, 127 lines of F2:10 and F2:11 soybean recombinant inbred lines constructed by Dongnong 46 and L-100 were used as mapping populations to develop bin markers and construct a genetic linkage map. The inclusive complete interval mapping(ICIM) method in IciMapping 4.0 software was used for analyzing the additive effect QTLs and the interaction of additive and epistatic QTLs for seed shape traits and 100-seed weight at four locations in two years. The results showed that 81 QTLs related to soybean seed shape traits and 100-seed weight were detected and distributed on 18 chromosomes, with the genetic contribution rate of 1.66%-30.70%. The QTLs with the highest contribution rate of the five traits were qSL-4-2(23.85%), qSW-1-1(15.40%), qST-1-2(17.66%), qSV-15-1(30.70%)and q100-SW-19-1(15.43%), respectively. A total 43 pairs of QTLs with additive×additive interactions for soybean seed shape traits and 100-seed weight were detected by additive×additive epistasis analysis,with the genetic contribution rate of 1.41%-23.19%.