大豆GmRLP19基因克隆及胁迫应答模式分析

以大豆品种Williams82为试验材料,克隆GmRLP19基因,通过生物信息学分析qPCR检测探讨该基因不同生育期品种、不同组织、不同非生物胁迫下表达模式。结果表明,该基因编码区全长3 138 bp,编码1 045个氨基酸,编码蛋白具有胞外信号肽,LRRNT域,富含亮氨酸重复结构域,跨膜结构域和一段胞内短肽,属于受体类蛋白家族,定位于质膜。该基因在不同生育期品种间差异表达,在豆和豆荚中特异性表达,且在5种非生物胁迫中均有响应。研究为探究WRKY20基因功能及挖掘和完善基因调控通路奠定基础。

Cloning of soybean GmRLP19 gene and analysis of stress response patterns

In this paper, the cloning and bioinformatics analysis of the gene was carried out using the soybean(Glycine max) variety Williams82. The expression pattern of the gene at different growth stages, different tissues and different abiotic stresses was further investigated by qPCR. The results showed that the coding region of this gene was 3 138 bp in length and encoded 1 045 amino acids. The encoded protein had an extracellular signal peptide, LRRNT domain, leucine-rich repeat domain,transmembrane domain and a short intracellular peptide. It belonged to the family of RLP and was localized to the plasma membrane. The gene was differentially expressed at different growth stages,specifically expressed at beans and pods, and responded in five abiotic stresses. The research laid the foundation for further exploring the function of the gene and excavating and perfecting the WRKY20 gene regulatory pathway.