| MAP30基因转化烟草的研究 |
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| 引用本文： | 栾杰,陈秀玲,侯莉华,李小梅,王傲雪.MAP30基因转化烟草的研究[J].东北农业大学学报,2012(7):109-113. |
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| 作者姓名： | 栾杰 陈秀玲 侯莉华 李小梅 王傲雪 |
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| 作者单位： | 1. 东北农业大学园艺学院,哈尔滨150030
2. 大庆市教师进修学院,黑龙江大庆163311 |
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| 基金项目： | 高等学校博士学科点专项科研基金(20092325110001);教育部科学技术研究重点项目(211043) |
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| 摘 要： | 通过转基因技术,利用农杆菌介导法,将pBIMAP30植物表达载体转化烟草,Kan筛选抗性苗,选择植株健壮、根系发达的抗性植株,提取叶片DNA进行PCR鉴定,获得阳性转基因烟草.采用RT-PCR方法证实MAP30基因能够在转录水平上正常表达.该研究为探讨具有药用价值的植物资源奠定理论基础,并为获得抗病毒、抗肿瘤功能性药物蛋白的植物新品系研发提供一定的技术支持
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| 关 键 词： | MAP30基因 烟草 农杆菌介导法 |
Study on transformation of MAP30 gene in tobacco |
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| LUAN Jie,CHEN Xiuling,HOU Lihua,LI Xiaomei,WANG Aoxue.Study on transformation of MAP30 gene in tobacco[J].Journal of Northeast Agricultural University,2012(7):109-113. |
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| Authors: | LUAN Jie CHEN Xiuling HOU Lihua LI Xiaomei WANG Aoxue |
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| Institution: | 1(1.School of Horticulture,Northeast Agricultural University,Harbin 150030,China;2.Daqing Teachers’ College for Vocational Studies,Daqing Heilongjiang 163311,China) |
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| Abstract: | The MAP30 gene was introduced into tobacco cultivar by Agrobacterium tumefaciens-mediated transformation,and the transformation system was optimized,and then the transgenic plants were detected.The result of PCR and RT-PCR showed the gene MAP30 was expressed in transgenic plants,which indicated that MAP30 gene was transformed into tobacco.The transgenic plant could be used to produce MAP30 for treatments of anti-virus and anti-tumor in the future. |
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| Keywords: | MAP30 tobacco Agrobacterium-mediated |
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