| 黑木耳原生质体制备及再生的研究 |
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| 引用本文： | 李楠,许修宏.黑木耳原生质体制备及再生的研究[J].东北农业大学学报,2009,40(7). |
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| 作者姓名： | 李楠 许修宏 |
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| 作者单位： | 东北农业大学资源与环境学院,哈尔滨,150030 |
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| 基金项目： | 中国科学院知识创新工程重大项目(KSCX1-YW-09-09-03);;哈尔滨市科技攻关计划项目(2007AA6CN105);;东北农业大学创新团队发展计划项目(CXT003-2-1) |
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| 摘 要： | 文章用浓度为0.6mol·L-1的MgSO4·7H2O渗透压稳定剂配制浓度为1.5%的溶壁酶,在pH6.0,30℃条件下酶解3h,黑木耳原生质体产量达到最高值为9.2×106个·mL-1。最适合原生质体再生的培养基配方为:马铃薯20%,葡萄糖2%,MgSO4·7H2O0.15%,蛋白胨0.1%,KH2PO40.1%,琼脂2%,甘露醇0.6mol·L-1,在此培养基中的再生率为33.09%。
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| 关 键 词： | 原生质体 原生质体再生 黑木耳 |
Protoplast preparation and regeneration of Auricularia auricula 29 |
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| LI Nan,XU Xiuhong.Protoplast preparation and regeneration of Auricularia auricula 29[J].Journal of Northeast Agricultural University,2009,40(7). |
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| Authors: | LI Nan XU Xiuhong |
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| Institution: | College of Resources and Environmental Sciences;Northeast Agricultural University;Harbin 150030;China |
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| Abstract: | The highest yield of protoplast could be obtained by using 1.5% lywallzyme made by 0.6 mol·L-1 MgSO4·7H2O), digesting for 3 h at the condition of 30℃ and pH value 6.0, the production of protoplast reached 9.2×106 number·mL-1. The formula of optimal medium for regeneration of Auricularia auricula was potato 20% glucose 2%, KH2OO4 0.1%, MgSO4·7H2O) 0.15%, peptone 0.1%, mannitol 0.6 moI·L-1, and agar 2%. The highest regeneration rate was 33.09%. |
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| Keywords: | protoplast protoplast regeneration Auricularia auricula |
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