| 常温高效纤维素分解菌的筛选 |
| |
| 引用本文： | 马怀良,郭文学,柴军红.常温高效纤维素分解菌的筛选[J].东北农业大学学报,2010,41(1). |
| |
| 作者姓名： | 马怀良 郭文学 柴军红 |
| |
| 作者单位： | 牡丹江师范学院生物系,黑龙江,牡丹江,157012;牡丹江市绿珠果蔬有限公司,黑龙江,牡丹江,157000 |
| |
| 基金项目： | 国家“十一五”科技支撑计划(2007BAD89B05-13) |
| |
| 摘 要： | 从长期堆放稻草的土壤中分离出12株常温纤维素分解菌。采用Hutchison液体培养基,对12株纤维素分解菌进行发酵产酶试验。结果表明,通过纤维素酶活力测定,最终筛选出对纤维素分解能力较强的霉菌菌株NM5,其C1酶活力、羧甲基纤维素酶活力、滤纸酶活力分别为0.0170、0.7174、1.3435IU·mL-1。根据菌落特征和形态特征,将NM5鉴定为木霉菌属(Trichoderma)。
|
| 关 键 词： | 纤维素分解菌 纤维素酶 常温 筛选 |
Screening high-performance cellulose-degrading microbes at normal temperature |
| |
| MA Huailiang,GUO Wenxue,CHAI Junhong.Screening high-performance cellulose-degrading microbes at normal temperature[J].Journal of Northeast Agricultural University,2010,41(1). |
| |
| Authors: | MA Huailiang GUO Wenxue CHAI Junhong |
| |
| Institution: | 1.Department of Biology;Mudanjiang Normal College;Mudanjiang Heilongjiang 157012;China;2.Mudanjiang Green-ball Fruits and Vegetables Co.;Ltd.;Mudanjiang Heilongjiang 157000;China |
| |
| Abstract: | Total 12 cellulolytic strains under the normal temperature were isolated from the soil that stacked the straw for a long term. The enzyme production experiments were carried out in Hutchison liquid medium and NM_5, which had the strongest cellulose decomposing ability among 12 cellulose decompos-ing strains was screened. The results showed that the C_1 enzyme activity, CMCase activity and FPA(filter paper activity) of the strain NM_5 was 0.0170, 0.7174 and 1.3435 IU·mL~(-1), respectively. According to the colonial and morphological characteristics, strain NM_5 was identified as Trichoderma. |
| |
| Keywords: | cellulose decomposition bacteria microbes cellulase normal temperature screening |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
