| 猪细小病毒PPV-SD1株VP2基因重组腺病毒表达载体的构建 |
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| 引用本文： | 王金良,沈志强,管宇,曲光刚,唐娜.猪细小病毒PPV-SD1株VP2基因重组腺病毒表达载体的构建[J].东北农业大学学报,2008,39(8). |
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| 作者姓名： | 王金良 沈志强 管宇 曲光刚 唐娜 |
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| 作者单位： | 山东省滨州畜牧兽医研究院,山东省畜禽用蜂胶疫苗工程技术研究中心,山东绿都生物科技有限公司,山东滨州256600 |
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| 摘 要： | 通过细菌内同源重组的方法成功构建了含有猪细小病毒(PPV)VP2基因的重组腺病毒表达载体。将VP2基因亚克隆连接到腺病毒穿梭载体pShuttle-CMV上,并将重组质粒pShuttle-CMV/VP2用Pme I线性化后转化至携带有腺病毒骨架载体pAdEasy-1的大肠杆菌BJ5183感受态细胞,经细菌内同源重组产生重组腺病毒质粒pAdEasy-VP2,为进一步研究PPV腺病毒活载体疫苗奠定了基础。
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| 关 键 词： | 猪细小病毒 VP2基因 重组腺病毒 |
Construction and identification of recombinant adenovirus containing porcine parvovirus PPV-SD1 strain VP2 gene |
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| WANG Jinliang,SHEN Zhiqiang,GUAN Yu,QU Guanggang,TANG Na.Construction and identification of recombinant adenovirus containing porcine parvovirus PPV-SD1 strain VP2 gene[J].Journal of Northeast Agricultural University,2008,39(8). |
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| Authors: | WANG Jinliang SHEN Zhiqiang GUAN Yu QU Guanggang TANG Na |
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| Abstract: | Recombinant replication-defective human adenovirus serotype 5 vector containing porcine parvo-virus(PPV) VP2 gene was constructed using the method of homologous recombination in bacteria.Firstly,VP2 gene was subcloned into adenovirus shuttle vectors.And then the positive recombinant plasmid pShuttle-CMV/VP2 was linearized by Pme I and carried into Ad-BJ5183 competent cells that had already been transformed with adenovirus skeletal vectors pAdEasy-1,the recombinant adenovirus plasmid pAdEasy-VP2 were successfully obtained.These results indicated that recombinant adenovirus could be used on animals for PPV recombinant adenovirus vaccine. |
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| Keywords: | porcine parvovirus VP2 gene recombinant adenovirus |
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