| 转CBF3基因烟草的抗寒性研究 |
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| 引用本文： | 刘荣梅,李凤兰,胡国富,胡宝忠.转CBF3基因烟草的抗寒性研究[J].东北农业大学学报,2011,42(1):119-123. |
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| 作者姓名： | 刘荣梅 李凤兰 胡国富 胡宝忠 |
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| 作者单位： | 东北农业大学生命科学学院;黑龙江省农业科学院植物脱毒苗木研究所; |
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| 基金项目： | 高等学校博士点专项科研基金(200802240008) |
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| 摘 要： | 为了解拟南芥转录因子CBF3基因转化烟草的抗寒性,利用农杆菌介导法将由诱导型启动子RD29A调控的CBF3基因导入烟草,获得Southern阳性转基因烟草;将转基因烟草幼苗及对照(未转基因烟草)在4℃低温胁迫8h,转基因烟草幼苗杭寒性强于对照;不同温度处理下转基因烟草及对照中渗透调节物质测定结果表明,低温处理下对照及转...
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| 关 键 词： | 低温胁迫 转基因烟草 可溶性糖 可溶性蛋白质 游离脯氨酸 |
Cold resistance of transgenic tobacco with CBF3 gene |
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| LIU Rongmei,LI Fenglan,HU Guofu,HU Baozhong.Cold resistance of transgenic tobacco with CBF3 gene[J].Journal of Northeast Agricultural University,2011,42(1):119-123. |
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| Authors: | LIU Rongmei LI Fenglan HU Guofu HU Baozhong |
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| Institution: | LIU Rongmei1,2,LI Fenglan1,HU Guofu1,HU Baozhong1(1.College of Life Sciences,Northeast Agricultural University,Harbin 150030,China,2.Institute of Virus-free Seedling Research,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China) |
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| Abstract: | In order to know stress tolerance of tobacco,the plant expression vector pBIRCBF3 including inducible promoter RD29A regulatory CBF3 gene were introduced to tobacco through Agrobacterium mediate method,and obtained resistant plants;Southern identity proved that pBIRCBF3 plant expression vector had already transferred to tobacco.When transgenic tobacco seedling and the control(normal tobacco seedling) were treated at the low temperature for 8 h,the cold resistance of transgenic tobacco seedling was better th... |
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| Keywords: | low temperature stress transgenic tobacco soluble sugar soluble protein free proline |
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