| 转DREB1A基因提高烟草抗逆性的研究 |
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| 引用本文： | 李晶,刘煜,蒿连梅,朱延明.转DREB1A基因提高烟草抗逆性的研究[J].东北农业大学学报,2011,42(1):124-129. |
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| 作者姓名： | 李晶 刘煜 蒿连梅 朱延明 |
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| 作者单位： | 东北农业大学生命科学学院; |
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| 基金项目： | 黑龙江省教育厅科技项目(10541294) |
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| 摘 要： | 将拟南芥(Arabidopsis thatiana)DREBIA基因分别在组成型启动子花椰菜病毒35S启动子和逆境诱导型启动子rd29A的驱动下转入烟草中,获得的两种转基因植株对干早和低温胁迫的抗性均显著提高,但对盐胁迫的杭性无明显变化.研究分析了组成型启动子和逆境诱导型启动子控制的DREBIA转基因植株在形态发育和抗...
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| 关 键 词： | DREB1A 烟草 抗逆性 启动子 遗传转化 |
Study on improving stress tolerance ability of tobacco(Nicotiana tabacum L.)using DREB1A gene |
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| LI Jing,LIU Yu,HAO Lianmei,ZHU Yanming.Study on improving stress tolerance ability of tobacco(Nicotiana tabacum L.)using DREB1A gene[J].Journal of Northeast Agricultural University,2011,42(1):124-129. |
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| Authors: | LI Jing LIU Yu HAO Lianmei ZHU Yanming |
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| Institution: | LI Jing,LIU Yu,HAO Lianmei,ZHU Yanming(College of Life Sciences,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | Arabidopsis thaliana DREB1A gene driven by constitutive promoter CaMV 35S and stress inducible promoter rd29A were respectively transformed into tobacco(Nicotiana tabacum L.).The obtained transgenic plants using both promoters showed strong drought and cold tolerance,but no salt tolerance was observed.The differences of characteristics between transgenic plants with DREB1A gene under the controlling of constitutive promoter and inducible promoter were analyzed,this will provide the technical basis for utili... |
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| Keywords: | DREB1A tobacco(Nicotiana tabacum L ) stress tolerance promoter genetic transformation |
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