| 大豆疫霉(Phytophthora sojae)单卵孢株毒性纯系的建立 |
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| 引用本文： | 杨明秀,文景芝,李永刚,刘春来,李莹.大豆疫霉(Phytophthora sojae)单卵孢株毒性纯系的建立[J].东北农业大学学报,2005,36(6):702-706. |
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| 作者姓名： | 杨明秀 文景芝 李永刚 刘春来 李莹 |
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| 作者单位： | 东北农业大学农学院,黑龙江,哈尔滨,150030;东北农业大学农学院,黑龙江,哈尔滨,150030;黑龙江省农科院植保所,黑龙江,哈尔滨,150086 |
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| 基金项目： | 黑龙江省博士后科研启动基金 |
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| 摘 要： | 将培养30d的大豆疫霉1号生理小种卵孢子在适宜条件下诱导萌发,随机挑取30个单卵孢株进行纯培养和毒力测定,在测定结果与亲代一致(1号生理小种)的菌株中随机选取1个单卵孢株重复上述试验。试验经过连续两代单卵孢株分离培养,获得了毒力稳定表达的大豆疫霉1号生理小种单卵孢株毒力纯系,为开展大豆疫霉毒性遗传和变异的分子生物学研究提供了研究材料。
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| 关 键 词： | 大豆疫霉 纯系 毒力 单卵孢株 |
| 文章编号： | 1005-9369（2005）06-0702-05 |
| 收稿时间： | 2004-11-22 |
| 修稿时间： | 2004年11月22 |
Development of virulent pure line of Phytophthora sojae through single-oospore progeny segregation |
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| YANG Ming-xiu,WEN Jing-zhi,LI Yong-gang,LIU Chun-lai,LI Ying.Development of virulent pure line of Phytophthora sojae through single-oospore progeny segregation[J].Journal of Northeast Agricultural University,2005,36(6):702-706. |
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| Authors: | YANG Ming-xiu WEN Jing-zhi LI Yong-gang LIU Chun-lai LI Ying |
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| Institution: | 1. Agriculture College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC; 2. Plant Protection Institute, Heilongjiang Academy of Agricultural Sciences, Harbin Heilongjiang 150086, PRC |
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| Abstract: | 30 single-oospore isolates obtained from race 1 of Phytophthora sojae(Ps-H2 strain) were evaluated using hypocotyls-inoculation method for race phenotype on soybean cultivars.One race 1 line with desired phenotypes was selected randomly for further experimentation.The pure-line in virulence was obtained after the second generation.This study demonstrated that the pure-breeding race of P.sojae could be developed by repeatedly self-fertilizing of oospore progeny.From this study,we got molecular research material of Phytophthora sojae on virulent inheritance and variation. |
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| Keywords: | Phytophthora sojae pure-line virulence single-oospore |
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