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荧光分光光度法测定大鼠不同脑区单胺类神经递质
引用本文:张燕,王洪斌,杨海霞,陈清,赵丽.荧光分光光度法测定大鼠不同脑区单胺类神经递质[J].东北农业大学学报,2010,41(1).
作者姓名:张燕  王洪斌  杨海霞  陈清  赵丽
作者单位:东北农业大学动物医学学院,哈尔滨,150030;东北农业大学生命科学学院,哈尔滨,150030
基金项目:国家自然科学基金项目(30671552);;黑龙江省自然科学基金重点项目(ZJN0503-01)
摘    要:介绍一种测定大鼠不同脑区单胺类神经递质的方法。采用荧光分光光度法同时测定大鼠不同脑区NE、DA和5-HT三种单胺类神经递质的含量。结果表明,荧光强度与浓度呈线性相关,NE、DA和5-HT的检测回收率分别为102.2%、95.4%和102.8%。该方法快速、灵敏、重复性较高,可为临床监测及科研提供参考。

关 键 词:荧光分光光度法  不同脑区  单胺类神经递质

Content determination of monoamine neurotransmitter in different encephalic regions in rat by fluorospectrophotometry
ZHANG Yan,WANG Hongbin,YANG Haixia,CHEN Qing,ZHAO Li.Content determination of monoamine neurotransmitter in different encephalic regions in rat by fluorospectrophotometry[J].Journal of Northeast Agricultural University,2010,41(1).
Authors:ZHANG Yan  WANG Hongbin  YANG Haixia  CHEN Qing  ZHAO Li
Institution:1.College of Veterinary Medicine;Northeast Agricultural University;Harbin 150030;China;2.College of Life Sciences;China
Abstract:The paper introduced a method for the determination of the monoamine neurotrans-mitter in different encephalic regions in rat.The contents of three monoamine neurotransmitter NE,L-DA and 5-HT in different encephalic regions in rat were measured by fluorospectrophotometry.The results showed that a linear dependence was presented between the fluorescence intensity and the concentration,the recovery rates of the NE,DA and 5-HT were 102.2%,95.4% and 102.8%,respectively.Thus,this method is rapid,sensitive and hi...
Keywords:fluorospectrophotometry  different encephalic regions  monoamine neurotransmitter  
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