| 甘蓝夜蛾核型多角体病毒某些生化特性的测定 |
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| 引用本文： | 倪艳松,张履鸿.甘蓝夜蛾核型多角体病毒某些生化特性的测定[J].东北农业大学学报,1992(2). |
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| 作者姓名： | 倪艳松 张履鸿 |
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| 作者单位： | 东北农学院植物保护系 哈尔滨150030 |
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| 摘 要： | 本文报道不连续系统的 SDS-PAGE 法对甘蓝夜蛾核型多角体病毒(Mamestrabrassicae nuclear polyhedrosis virus MbNPV)的多角体蛋白,病毒粒子结构多肽进行分析。结果表明,经热处理和温和碱解的多角体蛋白呈现一条带,分子量为28.2×10~3±500道尔顿。病毒粒子至少有15种多肽.MbNPV 多角体蛋白富含天门冬氨酸、谷氨酸。未检出色氨酸、限制性内切酶 ECoR Ⅰ,HindⅢ,BamH Ⅰ和 Pst Ⅰ分别把 MbNPV-DNA 酶解成15,13,7和10个限制性片段.由片段积加法计算得平均分子量为95.905±2.164Kbp.
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| 关 键 词： | 甘蓝夜蛾核型多角体病毒 聚丙烯酰胺凝胶电泳 限制性内切酶 多肽 脱氧核糖核酸 |
SOME BIOCHEMICAL CHARACTERISTICS OF THE NUCLEOPOLYHEDROSIS VIRUS OF CABBAGE MOTH(Mamestra brassicae L.) |
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| Ni Yansong Zhang Luhong.SOME BIOCHEMICAL CHARACTERISTICS OF THE NUCLEOPOLYHEDROSIS VIRUS OF CABBAGE MOTH(Mamestra brassicae L.)[J].Journal of Northeast Agricultural University,1992(2). |
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| Authors: | Ni Yansong Zhang Luhong |
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| Abstract: | The structural polypeptides of Mamestra brassicae nuclear polyhedrosis virus were stud-
ied on SDS-polyacrylamide slab using the discontinuous buffer system.The results showed
that the polyhedron comprised one major polypeptide of molecular weight 28.2×10~3±500d.
The virus particles contained 15 polypeptides ranging in molecular weight from 12.0 kd to
87.5kd.
The result of restriction endonuclease analysis with EcorRI,Hind Ⅲ,Bam HI and Pst Ⅰ
demonastrated that MbNPV-DNA had an approximate molecular weight of 95.905±
2.164Kbp. |
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| Keywords: | Mamestra brassicae NPV SDS-PAGE restriction endonuclease analysis polypeptide DNA |
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