| 重组鸡γ-干扰素对H5亚型禽流感灭活苗的免疫增强作用 |
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| 引用本文： | 曾岩,任晓峰,蔡皓璠,刘明,牛泽,李广兴.重组鸡γ-干扰素对H5亚型禽流感灭活苗的免疫增强作用[J].东北农业大学学报,2010,41(10). |
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| 作者姓名： | 曾岩 任晓峰 蔡皓璠 刘明 牛泽 李广兴 |
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| 作者单位： | 东北农业大学动物医学学院,哈尔滨,150030;中国农业科学院哈尔滨兽医研究所,哈尔滨,150001 |
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| 基金项目： | 黑龙江省自然科学基金重点项目 |
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| 摘 要： | 为研究重组鸡γ-干扰素(Chicken interferonγ,ChIFN-γ)对禽流感灭活疫苗的免疫增强效果,使用重组鸡γ-干扰素作为免疫佐剂,联合禽流感灭活疫苗采用胸部肌肉注射的方式免疫SPF雏鸡,免疫后每周测定血清抗体效价、免疫器官T、B淋巴细胞转化水平,攻毒后计算攻毒保护率。结果表明,鸡γ-干扰素提高了血清抗体水平,免疫器官T、B淋巴细胞转化水平和攻毒保护率,与疫苗组相比多数指标差异显著(P0.05)。鸡γ-干扰素对H5亚型禽流感灭活疫苗具有免疫增强作用。
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| 关 键 词： | 鸡γ-干扰素 禽流感病毒 灭活苗 免疫增强 |
Trial on efficacy of Al H5 type inactivated vaccine enhanced by recombinant chicken interferon γ- |
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| ZENG Yan,REN Xiaofeng,CAI Haofan,LIU Ming,NIU Ze,LI Guangxing.Trial on efficacy of Al H5 type inactivated vaccine enhanced by recombinant chicken interferon γ-[J].Journal of Northeast Agricultural University,2010,41(10). |
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| Authors: | ZENG Yan REN Xiaofeng CAI Haofan LIU Ming NIU Ze LI Guangxing |
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| Abstract: | The study aimed to evaluate the adjuvant effect of recombinant chicken interferon γ(ChIFN-γ) on avian influenza virus(AIV) inactivited vaccine.Specific pathogen-free(SPF) chicken were immunized with AIV inactivited vaccine,ChIFN-γor the combination of ChIFN-γand AIV inactivited vaccine.The serum antibody titer and the number of the T/B lymphocytes in chicken were analzyed at a week interval post-immunization.The protection rate of the vaccines to the immunized chicken was calculated after AIV challenge.The results showed that ChIFN-γ had positive effects on the inactivited vaccine,since the adjvant enhanced the titer of serum specific antibody and increased the number of T/B lymphocytes in immunized chicken significantly(P<0.05).ChIFN-γshould be the useful immunore-gulator for avian influenza inactivated vacuine. |
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| Keywords: | chicken interferon-γ avian influenza virus inactivated vaccine immunopotentiation |
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