肉仔鸡个体间RAPD指纹的变异

以白羽肉仔鸡为研究材料,应用随机扩增多态DNA(RAPD)技术,利用已筛选出的17个随机引物对15个公仔鸡和15个母仔鸡个体间的遗传变异进行分析,实验结果表明,(1)17个引物在母鸡群共产生145个扩增片段,其中有102个是多态性片段,多态率为7034%;17个引物在公鸡群共产生了154个扩增片段,其中有92个是多态性片段,多态率为5974%。这些结果说明RAPD标记的多态性较高,作为一种DNA标记,它可以用于分析鸡群体的遗传结构;(2)公、母鸡群体内个体间的遗传相似系数分别为08621,08214;(3)可以利用RAPD技术检测肉鸡杂合群体内个体间的遗传变异程度,但必须使用大量的随机引物。

VARIATION OF RAPD FINGERPRINTINGS AMONG INDIVIDUALS IN BROILERS

The Broilers studied were from a commercial line of broiler chicken(AA).The genetic variations among 15 male broilers and 15 female broilers were detected respectively by the random amplified polymorphic DNA (RAPD) method with 17 primers selected previously.The experiment results showed that:(1)One hundere and two out of 145 RAPD fragments were polymophic ones,and the polymophic percent was 70.34% in female population. Ninety two out of 154 RAPD fragments were found being polymophic ones,and the polymophic percent was 59.74% in male population.These results indicated that RAPD marker was high in polymorphism,and it was suitable for the analysis of genetic structure in chicken population.(2)The average pairwise similarity indices within male and female population were 0.8621 ,0.821 4,respectively.(3)A large numbers of primer should be used in order to detect genetic variation among individuals within a heterozygous population.$$$$