二甲苯胺噻唑对家兔血液、脑脊液、垂体及下丘脑中β－内啡肽浓度的影响

用放射免疫分析法（ＲＩＡ）研究二甲苯胺噻唑（静松灵）对家兔血液、脑脊淮、垂体及下丘脑中β－内啡肽（β－Ｅｐ）浓度的影响。给家兔肌注静松灵（３４ｍｇ／ｋｇ）后，家兔血液及脑脊液中β－Ｅｐ在用药后１．５ｈ增至最高值，即由用药前的７２．０８±４．０５ｐｇ／ｍＬ增至２８９．５４±１０．０４（Ｘ±ＳＥ，ｎ＝５，Ｐ＜０．０１），升高了７５．１１％；脑脊液中β－Ｅｐ在注药后１ｈ增至最高值，即由５３３．４９±１６．５５ｐｇ／ｍＬ增至１９０８．８０±２８．３６ｐｇ／ｍＬ（Ｘ±ＳＥ，ｎ＝５，Ｐ＜０．０１）增高了７２．０８％，经统计分析差异极显著，而对照组，注射前后家兔血液及脑脊液中β－Ｅｐ浓度未见明显变化。给药后家兔垂体及下丘脑中β－Ｅｐ浓度与给药前比较是动态变化规律，垂体中β－Ｅｐ在给药后３０ｍｉｎ降至最低值，即由２４６．６５±４．４２ｐｇ／ｍｇ降至１２０．５０±８．０９ｐｇ／ｍｇ（Ｘ±ＳＥ，ｎ＝３，Ｐ＜０．０１）降低了５１．１５％，以后是回开趋势，给药后３ｈ增至最高值：３３８．６０±１４．７８ｐｇ／ｍｇ，增加了２７．２６％，经统计分析，差异极显著；下丘脑中β－Ｅｐ浓度在给药后３０ｍｉｎ降至最低值，即由３２．１４±１．３０ｐ?

THE EFFECTS OF XYLAZOLE ON THE CONCENTRATION OF β-ENDORPHIN IN PLASMA, CSF, PITUITARY AND HGPOTHALAMUS IN RABBITS

The effects of xylazole on the concentration of β-Ep in plasma,CSF,pituitary and gypothalamus in rabbits were studied by means of radioimmunoassay(RIA).The levels of β-Ep in plasma and CSF were fouad to be inekeased as compared with the levels of contrds after i.m.adminstration of xylazole.The maximum levels of β-Ep in plasma were found at 1.5h after adminstration, it increased by 75.11% from 72.08±4.05 pg/mL to 289.54±10.04 pg/mL(X±SE,n=5,P<0.01),the maximum levels of β-Ep in CSF were found at lh, it incrcosed by 72.08% from 533.49±16. 55pg/mL to 1908.80±28.36pg/mL(X±SE, n=5, P<0.01) Marked changes have been found in the levels of β-Ep in pituitary and hypolhalamus after i.m.adminstration of xylazole as compared with those of controls(zero time).Initially,the levels of β-Ep significantly deereased and the minimal levels were found at 30 min, the levels of β-Ep in pituitary fell by 51.15% from 246.65±4.42 pg/mg to 120.50± 8.09 pg/mg(X ±SE,n=3,P<0.01)the levels of β-Ep in hypothalamus fell by 65.59% from 32.14±1.30 pg/mg to 11.06±0.35 pg/mg(X±SE,n=3, P<0.01),then,they became to increase and the maximum levels were found at 3 h the levels of β-Ep in pituitary and hypothalamus inercased by 27.16% and 51.07%,respectively,over the levels of β-Ep at zero time.The results of the cxperiment showed that xylazole may indues the relcase and synthesise of β-Ep in rabbits,which may be one of the important factors caused analgesie action of xyiazole.