| 实时荧光定量PCR技术研究进展及其应用 |
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| 引用本文： | 陈旭,齐凤坤,康立功,李景富.实时荧光定量PCR技术研究进展及其应用[J].东北农业大学学报,2010,41(8). |
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| 作者姓名： | 陈旭 齐凤坤 康立功 李景富 |
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| 作者单位： | 东北农业大学园艺学院,哈尔滨,150030 |
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| 摘 要： | 实时荧光定量PCR技术是在PCR反应体系中加入荧光染料或荧光探针,利用荧光信号积累实时监测整个PCR反应进程,最后通过标准曲线对未知模板进行定量分析,具有操作简便、快速高效,高通量,而且高敏感性等特点,该技术在分子诊断、分子生物学研究、动植物检疫以及食品安全检测等方面有广泛的应用。文章对实时荧光定量PCR的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
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| 关 键 词： | 实时荧光定量PCR 分类 影响因素 应用 |
Advance and application of real-time fluorescent quantitative PCR |
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| CHEN Xu,QI Fengkun,KANG Ligong,LI Jingfu.Advance and application of real-time fluorescent quantitative PCR[J].Journal of Northeast Agricultural University,2010,41(8). |
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| Authors: | CHEN Xu QI Fengkun KANG Ligong LI Jingfu |
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| Institution: | CHEN Xu,QI Fengkun,KANG Ligong,LI Jingfu(College of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | Real-time fluorescent quantitative PCR, in PCR reaction system by adding fluorescent dyes or fluorescent probes, real-time monitoring of fluorescence signal accumulation process of the PCR reaction, standard curve by quantitative analysis of the unknown template, simple, fast, efficient, high-throughput and the characteristics of high sensitivity. The technology in molecular diagnostics, molecular biology, animal and plant quarantine and food safety testing has a wide range of applications. Article on the p... |
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| Keywords: | real-time fluorescentquantitative PCR classification factors application |
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